Edaravone protects against lung injury induced by intestinal ischemia/reperfusion in rat
Edaravone protects against lung injury induced by intestinal ischemia/reperfusion in rat
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DOI：
10.1016/j.freeradbiomed.2004.10.029
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发表时间：
2005-02-01
影响因子：
7.4
通讯作者：
Horikawa, S
中科院分区：
文献类型：
--
作者：
Ito, K;Ozasa, H;Horikawa, S
肠缺血/再灌注（I/R）是远端器官功能障碍发展的关键和触发事件，通常涉及肺。呼吸衰竭是肠I/R后常见的死亡原因和并发症。在这项研究中，我们研究了依达拉奉（3-甲基-1-苯基-2-吡唑啉-5-酮）对大鼠肠I/R所致肺损伤的预防作用。依达拉奉已被用于保护脑梗死患者的I/R损伤。当大鼠遭受180分钟的肠缺血时，观察到24小时内的高死亡率。在这种情况下，在再灌注开始前静脉注射依达拉奉以剂量依赖性方式降低死亡率。为了更详细地检查依达拉奉对肠I/R诱导的肺损伤的疗效，我们进行了120分钟的肠缺血，随后进行了120分钟的再灌注。与未经依达拉奉处理的I/R处理的大鼠肺相比，依达拉奉处理降低了肠I/R后肺中的中性粒细胞浸润、脂质膜过氧化和促炎细胞因子白细胞介素-6 mRNA的表达。组织学分析也表明依达拉奉的有效性。总之，依达拉奉可改善肠I/R诱导的肺损伤，从而降低死亡率。(C)2004年爱思唯尔公司All rights reserved.
Intestinal ischemia/reperfusion (I/R) is a critical and triggering event in the development of distal organ dysfunction, frequently involving the lungs. Respiratory failure is a common cause of death and complications after intestinal I/R. In this study we investigated the effects of edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one) on the prevention of lung injury induced by intestinal I/R in rats. Edaravone has been used for protection against I/R injury in patients with cerebral infarction. When rats were subjected to 180 min of intestinal ischemia, a high incidence of mortality was observed within 24 h. In this situation, intravenous administration of edaravone just before the start of reperfusion reduced the mortality in a dose-dependent manner. To examine the efficacy of edaravone on the lung injury induced by intestinal I/R in more detail, we performed 120 min of intestinal ischemia followed by 120 min of reperfusion. Edaravone treatment decreased the neutrophil infiltration, the lipid membrane peroxidation, and the expression of proinflammatory cytokine interleukin-6 mRNA in the lungs after intestinal I/R compared to the I/R-treated rat lungs without edaravone treatment. Histopathological analysis also indicated the effectiveness of edaravone. In conclusion, edaravone ameliorated the lung injury induced by intestinal I/R, resulting in a reduction in mortality. (C) 2004 Elsevier Inc. All rights reserved.