A NOVEL CLASS OF MADS BOX GENES IS INVOLVED IN OVULE DEVELOPMENT IN PETUNIA
A NOVEL CLASS OF MADS BOX GENES IS INVOLVED IN OVULE DEVELOPMENT IN PETUNIA
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DOI：
10.1105/tpc.7.10.1569
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发表时间：
1995-10-01
期刊：
影响因子：
11.6
通讯作者：
VANTUNEN, AJ
中科院分区：
文献类型：
--
作者：
ANGENENT, GC;FRANKEN, J;VANTUNEN, AJ
我们从矮牵牛中分离并鉴定了两个胚珠特异的MADS box基因，命名为花结合蛋白基因7和11。这两个基因的蛋白产物有90%的氨基酸序列相似。原位RNA杂交实验表明，在胚珠原基可见之前，这两个基因都在雌蕊发育的中心表达。在发育后期，只在胚珠中观察到杂交信号，表明这些基因参与了胚珠的形成。为了验证这一假设，我们培育了转基因矮牵牛，其中fbp7和fbp11的表达都受到共抑制的抑制。在这些转化子的子房中，在通常由胚珠占据的位置上发育出意大利面形状的结构。这些异常结构在形态和功能上与花柱和柱头组织相似。我们的结果表明，这些MADS盒基因属于一类新的MADS盒基因，参与矮牵牛胚珠的正常发育。
We isolated and characterized two ovule-specific MADS box cDNAs from petunia, designated floral binding protein (fbp) genes 7 and 11. The putative protein products of these genes have similar to 90% of their overall amino acid sequence in common. In situ RNA hybridization experiments revealed that both genes are expressed in the center of the developing gynoecium before ovule primordia are visible. At later developmental stages, hybridization signals were observed only in the ovules, suggesting that these genes are involved in ovule formation. To test this hypothesis, we raised transgenic petunia plants in which both fbp7 and fbp11 expression was inhibited by cosuppression. In the ovary of these transformants, spaghetti-shaped structures developed in positions normally occupied by ovules. These abnormal structures morphologically and functionally resemble style and stigma tissues. Our results show that these MADS box genes belong to a new class of MADS box genes involved in proper ovule development in petunia.