Hepatocyte Growth Factor Region Specifically Stimulates Gastrointestinal Epithelial Growth in Primary Culture
Hepatocyte Growth Factor Region Specifically Stimulates Gastrointestinal Epithelial Growth in Primary Culture
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肝细胞生长因子区域特异性刺激原代培养中的胃肠上皮生长
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
T. Masui
中科院分区：
文献类型：
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作者：
H. Fukamachi;M. Ichinose;S. Tsukada;N. Kakei;T. Suzuki;K. Miki;K. Kurokawa;T. Masui
肝细胞生长因子（HGF）刺激原代培养的胎鼠胃肠道上皮细胞生长，并有明显的剂量-反应关系。胃肠道不同部位上皮细胞对HGF促有丝分裂活性的反应不同，腺胃最敏感，其次是肠和前胃。HGF与其他生长因子在诱导上皮生长中的相互作用也因上皮细胞的类型而异，表明胃肠道中的区域特异性生长调节。用北方杂交和RT-PCR分析显示，HGF mRNA仅在胃肠道间质中表达，而在上皮中不表达，而c-met（HGF受体）基因在两种组织中均有表达。这些结果表明，胃肠道间充质分泌HGF，通过旁分泌机制刺激附着上皮细胞的生长，并且上皮细胞对HGF的反应受到区域特异性生长调节机制的控制。
Abstract Hepatocyte growth factor (HGF) stimulated the growth of fetal rat gastrointestinal epithelial cells in primary culture with a clear dose-response relationship The epithelial response to the mitogenic activity of HGF was different among the region of the gastro-intestinal tract; glandular stomach most responsive, followed by intestine and forestomach. The interaction of HGF with other growth factors in inducing the epithelial growth was also different depending on the type of the epithelial cells, indicating a region-specific growth regulation in the gastro-intestinal tract. Analyses using Northern blot and RT-PCR revealed that HGF mRNA was expressed only in mesenchymes but not in epithelia of the gastro-intestinal tract while expression of c-met (HGF receptor) gene was observed in both tissues. These results suggest that gastro-intestinal mesenchymes secrete HGF which stimulates the growth of attaching epithelial cells by a paracrine mechanism and that the epithelial response to HGF is controlled by a region-specific growth regulatory mechanism.