Aquaporins in complex tissues: distribution of aquaporins 1-5 in human and rat eye
Aquaporins in complex tissues: distribution of aquaporins 1-5 in human and rat eye
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DOI：
10.1152/ajpcell.1998.274.5.c1332
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发表时间：
1998-05-01
影响因子：
5.5
通讯作者：
Nielsen, S
中科院分区：
文献类型：
--
作者：
Hamann, S;Zeuthen, T;Nielsen, S
视觉涉及多种生理液体运动。在这里，我们通过免疫印迹、高分辨率免疫细胞化学和免疫电子显微镜定义了人和大鼠眼中水通道蛋白（AQP）水转运蛋白的细胞和亚细胞位点。 AQP3 在球结膜上皮和腺体中丰富，但在角膜上皮中仅微弱存在。相反，AQP5 在角膜上皮和泪腺腺泡顶膜中很突出。 AQP1 在巩膜成纤维细胞、角膜内皮和角膜细胞以及覆盖小梁网和施累姆氏管的内皮细胞中大量表达。尽管 AQP1 在睫状非色素上皮中含量丰富，但在睫状色素上皮中不存在。虹膜的后上皮和前上皮以及晶状体前上皮也含有大量的 AQP1，但 AQP0（晶状体的主要内在蛋白）在晶状体纤维细胞中表达。视网膜 Muller 细胞和星形胶质细胞表现出显着浓度的 AQP4，而神经元和视网膜色素上皮不表现出水通道蛋白免疫标记。这些研究证明了 AQP1、AQP3、AQP4 和 AQP5 在不同的眼上皮细胞中的选择性表达，预测了每种细胞在眼睛中发生水运动的复杂网络中的特定作用。
Multiple physiological fluid movements are involved in vision. Here we define the cellular and subcellular sites of aquaporin (AQP) water transport proteins in human and rat eyes by immunoblotting, high-resolution immunocytochemistry, and immunoelectron microscopy. AQP3 is abundant in bulbar conjunctival epithelium and glands but is only weakly present in corneal epithelium. In contrast, AQP5 is prominent in corneal epithelium and apical membranes of lacrimal acini. AQP1 is heavily expressed in scleral fibroblasts, corneal endothelium and keratocytes, and endothelium covering the trabecular meshwork and Schlemm's canal. Although AQP1 is plentiful in ciliary nonpigmented epithelium, it is not present in ciliary pigmented epithelium. Posterior and anterior epithelium of the iris and anterior lens epithelium also contain significant amounts of AQP1, but AQP0 (major intrinsic protein of the lens) is expressed in lens fiber cells. Retinal Muller cells and astrocytes exhibit notable concentrations of AQP4, whereas neurons and retinal pigment epithelium do not display aquaporin immunolabeling. These studies demonstrate selective expression of AQP1, AQP3, AQP4, and AQP5 in distinct ocular epithelia, predicting specific roles for each in the complex network through which water movements occur in the eye.