Astaxanthin attenuated the stress-induced intestinal motility disorder via altering the gut microbiota
Astaxanthin attenuated the stress-induced intestinal motility disorder via altering the gut microbiota
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虾青素通过改变肠道微生物群减轻压力引起的肠道蠕动障碍
DOI：
10.1024/0300-9831/a000756
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发表时间：
2022
影响因子：
2.3
通讯作者：
Itoh Yoshito
中科院分区：
文献类型：
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作者：
Yasuda Ritsu;Kamada Kazuhiro;Murakami Takaaki;Inoue Ryo;Mizushima Katsura;Hirose Ryohei;Inoue Ken;Dohi Osamu;Yoshida Naohisa;Katada Kazuhiro;Uchiyama Kazuhiko;Handa Osamu;Ishikawa Takeshi;Takagi Tomohisa;Konishi Hideyuki;Naito Yuji;Itoh Yoshito
肠道菌群和短链脂肪酸（SCFAs）被认为是肠易激综合征病理生理的关键因素。虾青素是一种类胡萝卜素，具有很强的抗氧化和抗炎活性。在这项研究中，我们检测了虾青素对肠道微生物群、scfa和促肾上腺皮质激素释放因子（CRH）诱导的肠道高运动性的影响。雄性Wistar大鼠（每组12只）分别饲喂含或不含0。02%（w/w）虾青素治疗4周，静脉给予CRH或生理盐水。注射后2 h计数粪球数量。然后处死大鼠，注射后3 h收集盲肠内容物。对照组注射CRH显著增加粪便数量（2.0 vs. 6.5, p= 0.028），虾青素组无显著增加粪便数量（1.0 vs. 2.2, p= 0.229）（每组n= 6）。与对照组相比，虾青素组盲肠菌群发生了显著变化。虾青素组乙酸（81.1 μmol/g vs. 103.9 μmol/g, p= 0.015）和丁酸（13.4 μmol/g vs. 39.2 μmol/g, p< 0.001）浓度显著低于对照组（n= 12 /组）。虾青素减轻crh诱导的肠道高运动性，改变肠道微生物群和scfa的组成。
Gut microbiota and short-chain fatty acids (SCFAs) are recognized as key factors in the pathophysiology of irritable bowel syndrome. Astaxanthin is a carotenoid with strong antioxidant and anti-inflammatory activities. In this study, we examined the effects of astaxanthin on gut microbiota-, SCFAs-, and corticotropin-releasing factor (CRH)-induced intestinal hypermotility. Male Wistar rats (n= 12 per group) were fed a diet with or without 0. 02%(w/w) astaxanthin for four weeks and CRH or saline was administered intravenously. The number of fecal pellets was counted 2 h after injection. Then the rats were sacrificed, and the cecal content were collected 3 h after injection. The number of feces was significantly increased by CRH injection in the control group (2.0 vs. 6.5; p= 0.028), but not in the astaxanthin group (1.0 vs. 2.2; p= 0.229)(n= 6 per group). The cecal microbiota in the astaxanthin group was significantly altered compared with that in the control group. The concentrations of acetic acid (81.1 μmol/g vs. 103.9 μmol/g; p= 0.015) and butyric acid (13.4 μmol/g vs. 39.2 μmol/g; p< 0.001) in the astaxanthin group were significantly lower than that in the control group (n= 12 per group). Astaxanthin attenuates CRH-induced intestinal hypermotility and alters the composition of gut microbiota and SCFAs.