GROWTH PHASE-DEPENDENT MODIFICATION OF RNA-POLYMERASE IN ESCHERICHIA-COLI
GROWTH PHASE-DEPENDENT MODIFICATION OF RNA-POLYMERASE IN ESCHERICHIA-COLI
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DOI：
10.1007/bf00290644
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发表时间：
1991-11-01
期刊：
影响因子：
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通讯作者：
ISHIHAMA, A
中科院分区：
文献类型：
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作者：
OZAKI, M;WADA, A;ISHIHAMA, A
在大肠杆菌培养物从指数生长到稳定期的过渡期间，发现预先存在的RNA聚合酶被转化成至少三种不同的全酶形式，其可以通过磷酸纤维素柱色谱法分离。 这三种全酶形式的相对水平根据细胞生长的阶段而变化。 改变固定相形式的RNA聚合酶显示启动子识别特性，这是不同的从指数增长的细胞的全酶。 酶重组实验表明，改变的启动子选择性是由于核心酶的修饰。 我们认为，RNA聚合酶的调节在基因表达从指数增长过渡到稳定期的过程中起着重要的作用。
During the transition of Escherichia coli cultures from exponential growth to stationary phase, the pre-existing RNA polymerase was found to be converted into at least three different holoenzyme forms, which could be separated by phosphocellulose column chromatography. The relative levels of these three holoenzyme forms changed depending on the phase of cell growth. The altered stationary phase forms of RNA polymerase showed promoter recognition properties that were different from those of the holoenzyme from exponentially growing cells. Enzyme reconstitution experiments showed that the altered promoter selectivity was due to modification of the core enzyme. We propose that modulation of RNA polymerase plays a role in the global switch of gene expression during the transition from exponential growth to stationary phase.