Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine.
Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine.
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编码流感血凝素的DNA作为禽流感疫苗的用途。
DOI：
10.1089/dna.1993.12.785
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发表时间：
1993
影响因子：
3.1
通讯作者：
Webster,RG
中科院分区：
文献类型：
--
作者：
Fynan,EF;Robinson,HL;Webster,RG
最近，我们证明了直接接种表达血凝素7(H7)的DNA可以免疫鸡抵抗致命的H7流感病毒的攻击。这些实验使用了基于缺陷逆转录病毒的载体来表达H7(P188)(Robinsonet al.，1993)。在这里，我们报告了使用基于非逆转录病毒的H7表达载体(pCMV/H7)的保护性免疫。与以前使用的基于逆转录病毒的载体不同，该载体不能作为感染源传播(由于表型与外源或内源病毒蛋白混合)。疫苗接种是通过三种途径(静脉、腹膜和肌肉注射)分别接种100μg氯化铯提纯的生理盐水中的pCMV/H7DNA来完成的。在两次免疫后，禽类被用致死量的H7亚型鸡流感病毒攻击鼻孔。五项独立疫苗试验的结果表明，大约60%的pCMV/H7DNA接种鸡获得了保护性免疫。相比之下，接种了对照DNA的鸡只有3%在致命的挑战中存活下来。
Recently, we demonstrated that direct inoculation of a hemagglutinin 7 (H7)-expressing DNA could vaccinate chickens against a lethal H7 influenza virus challenge. These experiments used a defective-retroviral-based vector to express H7 (p188) (Robinsonet al., 1993). Here, we report protective immunizations using a non-retroviral-based vector for H7 expression (pCMV/H7). Unlike the previously used retro viral-based vector, this vector cannot be transmitted as an infectious agent (as a consequence of phenotypic mixing with exogenous or endogenous virus proteins). Vaccination was accomplished by inoculating young, immunocompetent chickens by each of three routes (intravenous, intraperitoneal, and intramuscular) with 100 μg of cesium chloride-purified pCMV/H7 DNA in saline. After two immunizations, birds were challengedviathe nares with a lethal dose of a highly virulent chicken influenza virus of the H7 subtype. The results of five independent vaccine trials demonstrated protective immunizations in approximately 60% of the pCMV/H7 DNA-inoculated chickens. By contrast, only 3% of the chickens inoculated with control DNA survived the lethal challenge.