IMPROVED DETECTION OF ACID-MINE WATER STRESSED COLIFORM BACTERIA ON MEDIA CONTAINING CATALASE AND SODIUM PYRUVATE
IMPROVED DETECTION OF ACID-MINE WATER STRESSED COLIFORM BACTERIA ON MEDIA CONTAINING CATALASE AND SODIUM PYRUVATE
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DOI：
10.1139/m90-095
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发表时间：
1990-08-01
影响因子：
2.8
通讯作者：
BISSONNETTE, GK
中科院分区：
文献类型：
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作者：
CALABRESE, JP;BISSONNETTE, GK
两株指数相和固定相大肠杆菌的纯培养悬液在酸性矿井水(AMW)中暴露后，过氧化氢酶活性显著降低。在非选择性回收培养基中添加外源过氧化氢酶(500-2000U)或丙酮酸钠(0.05-5%)，与不添加这些添加剂的回收培养基相比，AMW胁迫的大肠杆菌的检测能力提高了12-465倍，而添加3，3‘-硫代二丙酸则未能提高回收率。此外，使用含有1000U过氧化氢酶或1.0%丙酮酸的选择性M-FC、mT7和M-Endo培养液的体外实验，除了含有丙酮酸的M-Endo外，对AMW应激细胞的检测也得到了改善。然后，通过膜过滤技术，使用适当修改的介质来分析受AMW影响的气流。添加过氧化氢酶、丙酮酸或两者的组合可显著提高粪便和总大肠菌群的回收率，而不促进非大肠菌群的生长。添加丙酮酸和(或)过氧化氢酶的平板计数琼脂增强了总异养菌的检测。这些发现表明，在标准回收介质中添加过氧化氢酶或丙酮酸可以改善受AMW影响的水中大肠菌群和总异养菌的检测。
Pure culture suspensions of two strains of exponential and stationary phase Escherichia coli exhibited significant reductions in catalase activity following exposure to acid mine water (AMW). The exogenous addition of catalase (500-2000 U) or sodium pyruvate (0.05-5%) to a nonselective recovery medium resulted in enhanced detection (12- to 465-fold) of AMW-stressed E. coli as compared with recovery on the medium lacking these supplements, whereas addition of 3,3''-thiodipropionic acid failed to improve recovery. Additional in vitro experiments utilizing selective M-FC, mT7, and M-Endo media containing 1000 U catalase or 1.0% pyruvate similarly resulted in improved detection of AMW-stressed cells, with the exception of M-Endo containing pyruvate. Appropriately modified media were then used to analyze an AMW-impacted stream by the membrane filtration technique. Addition of catalase, pyruvate, or a combination of both significantly improved recovery of fecal and total coliforms without promoting growth of noncoliforms. Supplementation of plate count agar with pyruvate and (or) catalase enhanced detection of total heterotrophs. These findings suggest that addition of catalase or pyruvate to standard recovery media may improve detection of coliform and total heterotrophic bacteria in AMW-impacted waters.