Human immunoglobulin kappa light chain genes of subgroups II and III.
Human immunoglobulin kappa light chain genes of subgroups II and III.
复制标题
亚组 II 和 III 的人免疫球蛋白 kappa 轻链基因。
DOI：
10.1093/nar/13.18.6499
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发表时间：
1985
影响因子：
14.9
通讯作者：
Zachau,HG
中科院分区：
文献类型：
--
作者：
Klobeck,HG;Meindl,A;Combriato,G;Solomon,A;Zachau,HG
首次报道了亚组II和亚组III的功能重排的VK基因(缩写为参考文献1)的完整序列。这些基因是从合成KII或KIII轻链的溶血样细胞系中克隆出来的，从抗VK亚群特异性抗血清的免疫化学分析中得到了证明。这些数据，加上KIV基因的序列(在所附的论文中描述)和以前发表的KI基因的序列，使得代表人类K轻链的四个已知V区亚群的基因的比较成为可能。VKII基因与VKI、VKIII和VKIV基因的区别在于前导序列中有一个更长的内含子：426个碱基对120-220个碱基对。使用来自KII和KIII基因以及来自各自上游区域的探针对人类DNA进行杂交实验，有助于确定亚组特定的探针和杂交条件。
The first complete sequences of functionally rearranged VKgenes (abbreviations ref.l) of subgroups II and III are reported. The genes have been cloned from lyraphoid cell lines synthesizing KII or KIII light chains as evidenced from immunochemical analyses with anti-VKsubgroup-specific antisera. These data, together with the sequence of a KIV gene (described in the accompanying paper) and those of previously published KI genes make possible a comparison of genes representative of the four known V region subgroups of human K light chains. The VKII gene is distinguished from the VKI, VKIII, and VKIV genes by a much longer intron within the leader sequence: 426 bp vs ca. 120–220 bp. Blot hybridization experiments with human DNA digests using probes from the KII and KIII genes and from the respective upstream regions help to define subgroup specific probes and hybridization conditions.