TAL1/SCL is expressed in endothelial progenitor cells angioblasts and defines a dorsal-to-ventral gradient of vasculogenesis
TAL1/SCL is expressed in endothelial progenitor cells angioblasts and defines a dorsal-to-ventral gradient of vasculogenesis
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DOI：
10.1006/dbio.1997.8751
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发表时间：
1997-12-01
影响因子：
2.7
通讯作者：
Little, CD
中科院分区：
文献类型：
--
作者：
Drake, CJ;Brandt, SJ;Little, CD
在本研究中，我们证实TAL1/SCL是转录因子螺旋-环-螺旋家族的成员，也是小鼠造血谱系的重要调节因子，在鸟类的内皮谱系中表达。使用TAL1/SCL抗体和Qh1抗体来检测血管发育的最早事件，Qh1抗体是鹌鹑内皮细胞的既定标记物。双重免疫荧光共聚焦显微镜分析表明：(I)TAL1/SCL在鹌鹑和鸡的内皮细胞中都有表达；(Ii)TAL1/SCL的表达先于Qh1表位的表达；(Iii)TAL1/SCL的表达定义了内脏中胚层中的原始细胞亚群，而不是Qh1。总体而言，这些数据表明TAL1/SCL阳性/QH1阴性细胞是血管母细胞。此外，使用TAL1/SCL表达作为内皮细胞谱系的标志，我们证明除了先前描述的头尾倾斜外，还存在背侧到腹侧的血管生成进展。(C)1997年学术出版社。
In this study we establish that TAL1/SCL, a member of the helix-loop-helix family of transcription factors, and an important regulator of the hematopoietic lineage in mice, is expressed in the endothelial lineage of avians. The earliest events of vascular development were examined using antibodies to TAL1/SCL, and the QH1 antibody, an established marker of quail endothelial cells. Analyses using double immunofluorescence confocal microscopy show that: (i) TAL1/SCL is expressed by both quail and chicken endothelial cells; (ii) TAL1/SCL expression precedes that of the QH1 epitope; and (iii) TAL1/SCL, but not QH1, expression defines a subpopulation of primordial cells within the splanchnic mesoderm. Collectively these data suggest that TAL1/SCL-positive/QH1-negative cells are angioblasts. Further, using TAL1/SCL expression as a marker of the endothelial lineage, we demonstrate that in addition to the previously described cranial-to-caudal gradient, there is a dorsal-to-ventral progression of vasculogenesis. (C) 1997 Academic Press.