Chromosomal evolution and phylogenetic considerations in cuckoos (Ayes, Cuculiformes, Cuculidae)
Chromosomal evolution and phylogenetic considerations in cuckoos (Ayes, Cuculiformes, Cuculidae)
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DOI：
10.1371/journal.pone.0232509
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发表时间：
2020-05-29
期刊：
影响因子：
3.7
通讯作者：
Correa de Oliveira, Edivaldo Herculano
中科院分区：
文献类型：
--
作者：
dos Santos, Michelly da Silva;Kretschmer, Rafael;Correa de Oliveira, Edivaldo Herculano
杜鹃有着悠久的分类困难历史。该目物种已成为基于骨学、行为学、生态学、形态学和分子数据的多项研究的主题。尽管如此，鹃形目与其他目物种之间的关系仍然存在争议。本工作采用比较染色体绘制的方法对鹃科两个物种Guira guira (Gmelin, 1788)和Piaya cayana (Linnaeus, 1766)进行了分析，以研究该类群的染色体进化，并进行该物种的首次染色体作图。我们的结果表明，G. guira 具有很高的染色体多样性，其中 2n = 76，涉及祖先同线性的裂变和融合事件，而 P. cayana 仅呈现裂变，这导致了 2n = 90 的高二倍体数量。有趣的是，这些物种之间没有共同的染色体重排。我们基于吉姆萨染色的结果与其他杜鹃的先前数据以及杜鹃形目姐妹类群（杜鹃形目、Musophagiformes 和 Opisthocomiformes）的分类单元进行了比较。细胞遗传学比较表明，杜鹃物种可以分为至少三个主要类群。此外，我们没有发现任何证据表明鹃形目与之前提出的姊妹类群接近。
The Cuckoos have a long history of difficult classification. The species of this order have been the subject of several studies based on osteology, behavior, ecology, morphology and molecular data. Despite this, the relationship between Cuculiformes and species of other orders remains controversial. In this work, two species of Cuculidae, Guira guira (Gmelin, 1788) and Piaya cayana (Linnaeus, 1766), were analyzed by means of comparative chromosome painting in order to study the chromosome evolution of this group and to undertake the first chromosome mapping of these species. Our results demonstrate high chromosomal diversity, with 2n = 76 in G. guira, with fission and fusion events involving ancestral syntenies, while P. cayana presented only fissions, which were responsible for the high diploid number of 2n = 90. Interestingly, there were no chromosomal rearrangements in common between these species. Our results, based on Giemsa staining, were compared with previous data for other cuckoos and also with taxa proposed as sister-groups of Cuculiformes (Otidiformes, Musophagiformes and Opisthocomiformes). Cytogenetic comparisons demonstrated that cuckoo species can be divided into at least three major groups. In addition, we found no evidence to place Cuculiformes close to the groups proposed previously as sister-groups.