MOLECULAR CHARACTERIZATION, IMMUNOHISTOCHEMICAL LOCALIZATION AND EXPRESSION OF A RIBOSOMAL PROTEIN L17 GENE FROM Apis cerana cerana
MOLECULAR CHARACTERIZATION, IMMUNOHISTOCHEMICAL LOCALIZATION AND EXPRESSION OF A RIBOSOMAL PROTEIN L17 GENE FROM Apis cerana cerana
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DOI：
10.1002/arch.20386
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发表时间：
2010-10-01
影响因子：
2.2
通讯作者：
Xu, Baohua
中科院分区：
文献类型：
--
作者：
Meng, Fei;Zhang, Liang;Xu, Baohua
核糖体蛋白L17 （RPL17）是大（60S）核糖体亚基的核心蛋白，在蛋白质合成中起重要作用。本文从中华蜜蜂中分离到一个RPL17基因，命名为AccRPL17。比对分析表明，acrpl17与其他已知的rpl17具有较高的同源性。此外，基因组序列分析显示，5个外显子被4个内含子分裂，并且第一个内含子的位置相对保守，定位在5'非翻译区。还审查了与发展有关的部分假定的顺式作用因素。实时荧光定量PCR结果显示，第5天幼虫mRNA表达量最高。同时，免疫组化定位显示，AccRPL17主要集中在五龄幼虫的肌肉组织、柱头、体壁和眼周。进一步的研究表明，AccRPL17可能参与对非生物胁迫的反应。这是一篇试图分析RPL17在中华蜜蜂中的表达和分布的报道。这些结果表明，AccRPL17可能在昆虫发育过程中发挥重要作用，并讨论了AccRPL17在参与非生物胁迫中的重要性。(c) 2010 Wiley期刊公司
Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreover, genomic sequence analysis revealed that five exons are splitted by four introns, and the position of the first intron is comparatively conservative, being localized in the 5' untranslated region. Partial putative cis-acting elements related to development were also examined. Quantitative real-time PCR showed that the highest mRNA level was detected in larvae on the fifth day. Simultaneously, immunohistochemical localization showed that AccRPL17 is primarily concentrated in muscular tissues, stigma, body wall, and the surrounding of the eye in the fifth-instar larvae. Further studies suggested that AccRPL17 might be involved in responses to abiotic stresses. This is a report attempting to analyze the expression and distribution of RPL17 in A. cerana cerana. These results indicated that AccRPL17 might play an important role in insect development, and the importance of AccRPL17 in participating in abiotic stresses is discussed. (c) 2010 Wiley Periodicals, Inc.