Microsatellite markers for European Daphnia

Microsatellite markers for European Daphnia
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DOI:
10.1111/j.1471-8286.2005.01218.x
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发表时间:
2006-06-01
期刊:
MOLECULAR ECOLOGY NOTES
影响因子:
--
通讯作者:
Schwenk, K
Schwenk, K
中科院分区:
其他
文献类型:
--
作者:
Brede, N;Thielsch, A;Schwenk, K

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本文对欧洲水蚤群的D. galeata、D. hyalina、D. rosea、D. cucullata和D. curvirostris进行了32个多态微卫星标记。微卫星标记要么从基因组文库中分离出来,要么根据已有的姊妹类群序列信息进行优化。跨物种测试表明,除了一个多态性标记外,所有多态性标记都适用于一个以上的物种,这使得可以进行种内和种间遗传研究,即群体结构、杂交事件和渐渗。
We present 32 polymorphic microsatellite markers for species of the European Daphnia longispina group: D. galeata, D. hyalina, D. rosea, D. cucullata and D. curvirostris. Microsatellite markers were either isolated from genomic libraries or optimized based on previously published sequence information of sister taxa. Cross-species tests revealed that all but one of the polymorphic markers are applicable to more than one species, which allows intra- and interspecific genetic studies on, i.e. population structure, hybridization events and introgression.