Antimicrobial properties of an orthodontic adhesive combined with cetylpyridinium chloride
Antimicrobial properties of an orthodontic adhesive combined with cetylpyridinium chloride
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DOI：
10.1016/j.ajodo.2005.10.015
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发表时间：
2006-02-01
影响因子：
3
通讯作者：
Wu, CD
中科院分区：
文献类型：
--
作者：
Al-Musallam, TA;Evans, CA;Wu, CD
简介：接受固定矫治器治疗的患者唾液和牙菌斑中变形链球菌水平增加的风险更大，龋齿的风险也更高。已知氯化十六烷基吡啶鎓（CPC）是一种有效的抗牙菌斑剂。本研究的目的是调查将 CPC 加入市售正畸粘合剂中是否会在不改变材料的直径拉伸强度的情况下赋予抗菌性能。方法：将不同量的 CPC 添加到市售的填充型光激活托槽粘合剂（Transbond XT，Unitek 3M，蒙罗维亚，加利福尼亚州）中，以获得 CPC 浓度为 0%（对照）、2.5%、5.0% 和 10.0%（重量）的样本。将厚度为 2 毫米、直径为 4 毫米的粘合盘与变形链球菌一起孵育 48 小时。在琼脂圆盘扩散测定中测量细菌抑制区域的直径；每个浓度的样本每隔一周进行一次测试，持续 196 天。其他光盘在蒸馏水中浸泡180天。使用分光光度计在 254 nm 处测量并记录第 7、15、30、60 和 180 天从改良圆盘释放到水中的 CPC 量。用万能试验机测量了改性粘合盘的径向拉伸强度，并评估了水老化的影响。结果：随着CPC含量的增加，测得的细菌抑制范围增加。所有 CPC 粘合剂样本均保持抗菌活性长达 196 天。在对照样本周围没有测量到细菌抑制区。观察到 180 天期间结束时的 CPC 释放情况，但最大释放量出现在第一周。 2.5% CPC 粘合剂组和对照组之间的径向拉伸强度没有显着差异 (P < .05)； 5.0%和10.0% CPC粘合剂组与对照组之间存在显着差异。水老化除了降低含有 10.0% CPC 的测试组的径向拉伸强度外，对径向拉伸强度没有显着影响。结论：在粘合剂材料中加入 2.5% CPC 可在不改变径向拉伸强度的情况下赋予抗菌活性。
Introduction: Patients undergoing fixed appliance treatment are at greater risk for increases in salivary and plaque levels of Streptococcus mutans and an elevated risk of dental caries. Cetylpyridinium chloride (CPC) is known to be an effective antiplaque agent. The purpose of this study was to investigate whether incorporating CPC into a commercially available orthodontic adhesive would impart antimicrobial properties without altering the diametral tensile strength of the material. Methods: CPC was added to a commercially available, filled, photo-activated bracket adhesive (Transbond XT, Unitek 3M, Monrovia, Calif) in varying amounts, to obtain specimens with CPC concentrations of 0% (control), 2.5%, 5.0%, and 10.0% by weight. Adhesive discs 2 mm thick and 4 mm in diameter were incubated with Streptococcus mutans for 48 hours. The diameters of the zones of bacterial inhibition were measured in an agar disc diffusion assay; specimens of each concentration were tested every other week for 196 days. Other discs were soaked in distilled water for 180 days. The amount of CPC released into the water from the modified discs was measured and recorded on days 7, 15, 30, 60, and 180 by using a spectrophotometer at 254 nm. Diametral tensile strength of the modified adhesive discs was measured with a universal testing machine, and the effect of water aging was also evaluated. Results: The measured zone of bacterial inhibition increased as CPC content increased. All CPC-adhesive specimens maintained antimicrobial activity up to 196 days. No zone of bacterial inhibition was measured around the control specimens. CPC release was observed through the end of 180-day period, but the greatest release was recorded in the first week. There was no significant difference (P < .05) in diametral tensile strength between the 2.5% CPC-adhesive group and the control; there were significant differences among the 5.0% and 10.0% CPC-adhesive groups and the control. Water aging had no significant effect on diametral tensile strength other than decreasing it for the test group containing 10.0% CPC. Conclusions: The incorporation of 2.5% CPC in adhesive material imparted antimicrobial activity without altering diametral tensile strength.