Automated behavioral analysis of mice using INTELLICAGE: inter-laboratory comparisons and validation with exploratory behavior and spatial learning
Automated behavioral analysis of mice using INTELLICAGE: inter-laboratory comparisons and validation with exploratory behavior and spatial learning
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发表时间：
2005
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通讯作者：
O. Litvin;M. Galsworthy;P. Zinn;F. Neuhäusser-wespy;H. Würbel;R. Nitsch
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作者：
O. Litvin;M. Galsworthy;P. Zinn;F. Neuhäusser-wespy;H. Würbel;R. Nitsch
INTELLICAGE TM 是一个大型家用笼子，包含四个放置在角落的完整操作调节单元。中央计算机在数小时、数天或数周内连续控制和监控每个笼子中多达 16 只带有应答器标签的小鼠的活动和学习，而无需人为干扰。小鼠行为表型分析的一个常见问题是实验室之间的遗传差异不一致。这部分是由于实验者之间无法控制的相互作用、实验设置的变化以及小鼠单独暴露于各种刺激和情况下的不同应激反应。在这里，我们比较了在相同条件下饲养的小鼠，然后在两个不同的实验室进行探索行为和空间学习测试，首先使用相同的测试设置进行手动测试，然后在自动化的 INTELLICAGE 中进行测试。这里提供的数据表明，自动化测试在各个实验室提供了更加一致的数据，并且表明，INTELLICAGE 测量的行为特征至少部分与小鼠探索行为和空间学习的标准测试中观察到的行为特征相同。
INTELLICAGE TM is a large home cage containing four complete operant conditioning units placed in the corners. A central computer continuously controls and monitors activity and learning of up to 16 transponder-tagged mice per cage without human interference over hours, days or weeks. A common problem in behavioral phenotyping of mice is that genetic differences are inconsistent across laboratories. This is partially due to uncontrollable interactions between experimenter, variations of experimental set-up and variable stress reactions of mice being exposed singly to a variety of stimuli and situations. Here we compare mice that have been raised under identical conditions but then tested in two different laboratories for exploratory behavior and spatial learning, first by manual testing using identical test set-ups, and then in the automated INTELLICAGE. The data presented here illustrate that automated testing provides much more consistent data across laboratories, and they show that INTELLICAGE measures at least partially the same behavioral traits as observed in standard tests of mouse exploratory behavior and spatial learning.