Characterization of the plasma lipoproteins of the genetically obese hyperlipoproteinemic Zucker fatty rat.
Characterization of the plasma lipoproteins of the genetically obese hyperlipoproteinemic Zucker fatty rat.
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DOI：
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发表时间：
1974-09
影响因子：
6.5
通讯作者：
G. Schonfeld;C. Felski;M. A. Howald
中科院分区：
文献类型：
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作者：
G. Schonfeld;C. Felski;M. A. Howald
对 Zucker 脂肪大鼠的血浆脂蛋白的脂质和脱辅基蛋白组成进行了表征，并将结果与瘦对照获得的结果进行了比较。关于脱辅基蛋白的信息通过凝胶过滤实验和聚丙烯酰胺凝胶电泳获得。脂肪中的极低密度脂蛋白 (VLDL) 增加了数倍，其质量的 78% 是甘油三酯，而对照组为 60%。低密度 (LDL) 和高密度 (HDL) 脂蛋白增加了 2 倍，尽管它们的成分与对照组相似。脂肪中的apoVLDL、apoLDL 和apoHDL 水平分别高出五倍、两倍和两倍，并且聚丙烯酰胺凝胶上移动最快的两个亚基肽在脱辅基蛋白中不成比例地升高。这两条带中速度较慢的一条在脂肪中的含量相对较快的一条要多。如果较慢的肽是脂蛋白脂肪酶的激活剂，类似于人类载脂蛋白的类似亚基肽，那么脂肪血浆中的脂肪酶激活剂活性可能比对照血浆高出10倍。这一发现以及脂肪组织脂蛋白脂肪酶活性约为对照的 150% 的事实表明，脂肪的 VLDL 分解代谢能力有所增强。我们之前已经表明，这些动物的肝脏 VLDL 分泌率高于正常水平。分解代谢能力的增加可能是对分泌率改变的反应。
The plasma lipoproteins of the Zucker fatty rat were characterized with respect to lipid and apoprotein composition, and results were compared with those obtained from lean controls. Information on apoproteins was obtained from gel filtration experiments and electrophoresis on polyacrylamide gels. Very low density lipoproteins (VLDL) were increased several-fold in fatties, and 78% of their mass was triglycerides compared with 60% in the controls. Low density (LDL) and high density (HDL) lipoproteins were increased by a factor of 2, although their compositions were similar to those of the controls. Levels of apoVLDL, apoLDL, and apoHDL were five, two and two times higher, respectively, in the fatties, and the two most rapidly moving subunit peptides on polyacrylamide gels were disproportionately elevated in the apoproteins. The slower of these two bands was present in relatively greater amounts than the faster one in fatties. If the slower peptide is an activator of lipoprotein lipase, analogous to the comparable subunit peptides of human apolipoproteins, plasmas of fatties could contain up to 10 times more lipase activator activity than control plasma. This finding, and the fact that adipose tissue lipoprotein lipase activity of fatties was about 150% of controls, suggests that fatties have increased capacities for VLDL catabolism. We have previously shown that hepatic VLDL secretory rates are higher than normal in these animals. The increased capacity for catabolism may be a response to the altered secretory rates.