A somatodendritic distribution of Rab11 in rabbit brain neurons.
A somatodendritic distribution of Rab11 in rabbit brain neurons.
复制标题
Rab11 在兔脑神经元中的体细胞树突分布。
DOI：
10.1097/00001756-199605170-00016
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发表时间：
1996
期刊：
影响因子：
1.7
通讯作者：
Goldenring,JR
中科院分区：
文献类型：
--
作者：
Sheehan,D;Ray,GS;Calhoun,BC;Goldenring,JR
神经元和上皮细胞的亚细胞靶向比较表明，顶端上皮抗原定位于轴突和神经末梢。我们研究了小的gtp结合蛋白Rab11在大脑中的分布，该蛋白定位于上皮细胞的顶端泡群。兔脑切片采用抗兔Rabll单克隆抗体免疫组化检测。Rabll免疫反应性仅存在于神经元中。在检查的所有区域，包括前脑、小脑、丘脑和脑干，在细胞体和树突中观察到Rabll免疫反应性。海马神经元未见染色。这些结果表明，Rabll的分布不支持先前认为顶端上皮标记应该定位于轴突和突触末梢的观点。
COMPARISONS of subcellular targeting in neurons and epithelial cells have led to suggestions that apical epithelial antigens localize to axons and nerve terminals. We have studied the distribution in brain of the small GTP-binding protein, Rab11, which is localized to apical vesicular populations in epithelial cells. Sections of rabbit brain were examined by immunohistochemistry using a monoclonal antibody against rabbit Rabll. Rabll immunoreactivity was present exclusively in neurons. In all regions examined, including forebrain, cerebellum, thalamus and brainstem, Rabll immunoreactivity was observed in cell bodies and dendrites. No staining was observed in hippocampal neurons. These results indicate that the distribution of Rabll does not support previous suggestions that apical epithelial markers should localize to axons and synaptic endings.