Dielectrophoretic traps for single-particle patterning
Dielectrophoretic traps for single-particle patterning
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DOI：
10.1529/biophysj.104.049684
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发表时间：
2005-03-01
影响因子：
3.4
通讯作者：
Voldman, J
中科院分区：
文献类型：
--
作者：
Rosenthal, A;Voldman, J
我们提出了一种新的微加工介电电泳陷阱设计图案的单细胞大阵列。因为.在细胞图案化过程中，去除未捕获的细胞通常是速率限制步骤，我们设计的捕获器足够坚固，以保持颗粒抵抗实际流速。我们通过测量聚苯乙烯珠在保持被捕获的同时可以承受的最大流速来实验验证捕获强度。这些珠实验已经显示出与我们的模型预测非常一致，而不使用拟合参数。该模型能够为我们提供陷阱如何工作的基本理解，并允许我们建立一套设计规则，用于优化各种细胞大小的陷阱。我们提供了一种使能技术的基础，该技术可用于以独特的方式对细胞进行图案化，使我们能够在微观尺度上进行新颖的细胞生物学实验。
We present a novel microfabricated dielectrophoretic trap designed to pattern large arrays of single cells. Because. owing away untrapped cells is often the rate-limiting step during cell patterning, we designed the trap to be strong enough to hold particles against practical flow rates. We experimentally validated the trap strength by measuring the maximum flow rate that polystyrene beads could withstand while remaining trapped. These bead experiments have shown excellent agreement with our model predictions, without the use of fitting parameters. The model was able to provide us with a fundamental understanding of how the traps work, and additionally allowed us to establish a set of design rules for optimizing the traps for a wide range of cell sizes. We provide the foundations for an enabling technology that can be used to pattern cells in unique ways, allowing us to do novel cell biology experiments at the microscale.