Photolytic interface for high-performance liquid chromatography--chemiluminescence detection of non-volatile N-nitroso compounds.
Photolytic interface for high-performance liquid chromatography--chemiluminescence detection of non-volatile N-nitroso compounds.
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DOI：
10.1039/an9891400155
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
James J. Conboy;J. H. Hotchkiss
中科院分区：
文献类型：
--
作者：
James J. Conboy;J. H. Hotchkiss
建立了一种高效液相色谱-化学发光检测器光解接口，用于生物样品中非挥发性N-亚硝基化合物的痕量检测。将含有分离的N-亚硝基氨基酸和N-亚硝基酰胺的色谱流出物引入具有He吹扫流的玻璃盘管中，并用紫外光照射。通过光解裂解的氮氧化物通过一系列冷阱从溶剂中快速分离，并由He携带到化学发光检测器的反应室中。该方法是兼容的大多数类型的HPLC，特别是反相，并产生低纳克的灵敏度未衍生的N-亚硝基氨基酸和N-亚硝基酰胺。证明了在加标的猪胃液（42微克l-1）中检测模型N-亚硝基酰胺，三甲基亚硝基脲，以及在加标的人尿液（7-8微克l-1）中检测N-亚硝基脯氨酸和N-亚硝基-1，3-噻唑烷-4-羧酸。
A photolytic interface between high-performance liquid chromatography (HPLC) and a chemiluminescence detector has been developed for the trace detection of non-volatile N-nitroso compounds in biological matrices. A chromatographic effluent containing separated N-nitrosoamino acids and N-nitrosamides is introduced into a glass coil with a purge stream of He and irradiated with ultraviolet light. Nitrogen oxide, cleaved by photolysis, is separated rapidly from the solvent through a series of cold traps and carried by the He into the reaction chamber of a chemiluminescence detector. The method is compatible with most types of HPLC, especially reversed-phase, and yields low-nanogram sensitivity for underivatised N-nitrosoamino acids and N-nitrosamides. The detection of a model N-nitrosamide, trimethylnitrosourea, in spiked porcine gastric fluid (42 micrograms l-1), and of N-nitrosoproline and N-nitroso-1,3-thiazolidine-4-carboxylic acid, in spiked human urine (7-8 micrograms l-1), is demonstrated.