Changes in cytosol and nuclear progesterone receptor concentrations in the rabbit uterus and their relation to induction of progesterone-regulated uteroglobin.
Changes in cytosol and nuclear progesterone receptor concentrations in the rabbit uterus and their relation to induction of progesterone-regulated uteroglobin.
复制标题
兔子宫中胞质和核孕酮受体浓度的变化及其与孕酮调节子宫珠蛋白诱导的关系。
DOI：
10.1016/0006-291x(79)91112-4
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发表时间：
1979
影响因子：
3.1
通讯作者：
O. Jänne
中科院分区：
文献类型：
--
作者：
V. Isomaa;H. Isotalo;M. Orava;T. Torkkeli;O. Jänne
为了研究类固醇受体浓度与生物反应之间的关系，我们在不同的实验条件下测量了兔子宫的胞质和核孕酮受体，并将受体值与孕酮调节蛋白子宫珠蛋白的诱导进行了比较。为期 5 天的黄体酮治疗（每天 1 毫克/公斤）使核受体含量降低了 40%，胞浆受体水平略有升高，子宫珠蛋白含量增加了 3000 倍。雌二醇和他莫昔芬改变了孕酮诱导的受体和子宫珠蛋白值的变化：当孕酮治疗补充雌二醇或他莫昔芬时，胞质和核受体显着上升，但子宫珠蛋白诱导显着下降。每天 1 μg/kg 的雌二醇可实现 50% 黄体酮对子宫珠蛋白合成的抑制。因此，在某些条件下，类固醇受体水平和生物反应之间存在明显差异。
To study the relation between steroid receptor concentrations and biological response, we measured cytosol and nuclear progesterone receptors from rabbit uterus under different experimental conditions, and compared receptor values with induction of uteroglobin, a progesterone-regulated protein. A 5-day progesterone treatment (1 mg/kg per day) reduced the nuclear receptor content by 40%, slightly elevated cytosol receptor levels and increased uteroglobin content 3000-fold. Estradiol and tamoxifen altered progesterone-induced changes in the receptor and uteroglobin values: cytosol and nuclear receptors rose significantly, but uteroglobin induction declined markedly, when progesterone treatment was supplemented with estradiol or tamoxifen. A 50% inhibition of progesterone action on uteroglobin synthesis was achieved with 1 μg/kg of estradiol per day. Thus under certain conditions, there is a clear disparity between steroid receptor levels and biological response.