Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line
Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line
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DOI：
10.1073/pnas.0605087103
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发表时间：
2006-08-08
影响因子：
11.1
通讯作者：
Nurminsky, Dmitry I.
中科院分区：
文献类型：
--
作者：
Mikhaylova, Lyudmila M.;Boutanaev, Alexander M.;Nurminsky, Dmitry I.
早期精母细胞的转录激活涉及数百个基因，其中许多是减数分裂和精子细胞分化所必需的。一些减数分裂阻滞基因已被确定为转录的一般调节因子，然而，基因特异性转录因子仍然难以捉摸。为了鉴定这些因子，我们纯化了特异性结合精子细胞分化基因Sdic启动子的蛋白质，并将其鉴定为核仁素的果蝇同源物Modulo。在雄性不育模突变体的基因表达模式的分析表明，模支持减数分裂阻滞基因的高表达，是精子细胞分化基因的转录所必需的。Modulo本身的表达受减数分裂阻滞基因的控制，并需要DAZ/DAZL同源物Boule参与G（2）/M转换的控制。因此，Modulo，Boule和减数分裂阻滞基因之间的调控相互作用整合了减数分裂和精子！雄性生殖细胞系的分化。
Transcriptional activation in early spermatocytes involves hundreds of genes, many of which are required for meiosis and spermatid differentiation. A number of the meiotic-arrest genes have been identified as general regulators of transcription; however, the gene-specific transcription factors have remained elusive. To identify such factors, we purified the protein that specifically binds to the promoter of spermatid-differentiation gene Sdic and identified it as Modulo, the Drosophila homologue of nucleolin. Analysis of gene-expression patterns in the male sterile modulo mutant indicates that Modulo supports high expression of the meiotic-arrest genes and is essential for transcription of spermatid-differentiation genes. Expression of Modulo itself is under the control of meiotic-arrest genes and requires the DAZ/DAZL homologue Boule that is involved in the control of G(2)/M transition. Thus, regulatory interactions among Modulo, Boule, and the meiotic-arrest genes integrate meiosis and spermatic! differentiation in the male germ line.