Phylogenetic analysis of the E2 gene of classical swine fever virus from the Guangxi Province of southern China
Phylogenetic analysis of the E2 gene of classical swine fever virus from the Guangxi Province of southern China
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DOI：
10.1007/s11262-011-0578-8
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发表时间：
2011-06-01
期刊：
影响因子：
1.6
通讯作者：
Zhang, Hong-Yun
中科院分区：
文献类型：
--
作者：
Luo, Ting Rong;Liao, Su-huan;Zhang, Hong-Yun
在这项研究中，从中国广西省的2001-2009年期间，从患有急性猪瘟的猪、流产的胎儿、1-2日龄死亡的新生猪、健康猪的扁桃体和免疫母猪的白细胞中采集了疑似猪瘟(CSF)样本。 RT-PCR检测775份样本中约92份呈阳性，获得41株分离株。通过E2基因测序对31个分离株进行系统发育分析，发现分离株分为两组：（1）流产胎儿（GXGZ02除外）、死亡新生仔猪、健康猪扁桃体和免疫母猪白细胞的分离株属于1.1组，另外还有疫苗株HCLV和中国石门标准毒株，以及(2)20株来自急性脑脊液猪的分离株属于2.1组，其中13株与来自中国其他省份的分离株归为2.1b亚组，其中7株与来自意大利和德国的分离株归为2.1a亚组。
In this study, suspected classical swine fever (CSF) samples from the Guangxi Province of China were obtained from pigs with acute CSF, aborted fetuses, newborn pigs that died at 1-2 days of age, tonsils of healthy pigs, and leukocytes of immunized sows during 2001-2009. About 92 of 775 samples were found to be positive by RT-PCR, and 41 isolates were obtained. Phylogenetic analysis was performed on the 31 isolates by sequencing the E2 gene, and the isolates were found to cluster into two groups: (1) isolates from aborted fetuses (except GXGZ02), deceased newborn baby pigs, tonsils of healthy pigs, and leukocytes of immunized sows belonged to group 1.1, along with vaccine strain, HCLV, and standard virulent strain, Shimen, of China, and (2) 20 isolates from pigs with acute CSF belonged to group 2.1, 13 of which were clustered into subgroup 2.1b with isolates from other provinces of China, and 7 of which were clustered into subgroup 2.1a with isolates from Italy and Germany.