Direct detection of renal function markers using microchip CE with pulsed electrochemical detection
Direct detection of renal function markers using microchip CE with pulsed electrochemical detection
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DOI：
10.1039/b403529a
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发表时间：
2004-01-01
期刊：
影响因子：
4.2
通讯作者：
Henry, CS
中科院分区：
文献类型：
--
作者：
Garcia, CD;Henry, CS
肌酸、肌酸和尿酸是在各种临床测定中测量的三种重要化合物，最值得注意的是用于肾功能。这些化合物的传统临床测定集中在酶或化学反应的使用上。电泳微芯片具有将毛细管电泳的分离能力与小型、便携且具有传统传感器的速度的设备集成的潜力。介绍了一种直接检测肌酐、肌酸和尿酸的微芯片毛细管电泳系统的研制。该设备使用脉冲安培检测（PAD）来检测含氮化合物以及易氧化的尿酸。使用30 mM硼酸盐缓冲液（pH = 9.4）在小于200 s内实现肌酸酐、肌酸和尿酸的基线分离。获得线性校准曲线，肌酐、肌酸和尿酸的检测限分别为80 mM、250 mM和270 mM。还示出了分离条件的优化和PAD与其他安培检测模式的比较。最后，分析了一个真实的尿液样本与验证的肌酐浓度使用的临床检测试剂盒的基础上的Jaffe反应。
Creatinine, creatine, and uric acid are three important compounds that are measured in a variety of clinical assays, most notably for renal function. Traditional clinical assays for these compounds have focused on the use of enzymes or chemical reactions. Electrophoretic microchips have the potential to integrate separation power of capillary electrophoresis with devices that are small, portable, and have the speed of conventional sensors. The development of a microchip CE system for the direct detection of creatinine, creatine, and uric acid is presented. The device uses pulsed amperometric detection (PAD) to detect the nitrogen-containing compounds as well as the easily oxidizable uric acid. Baseline separation of creatinine, creatine and uric acid was achieved using 30 mM borate buffer ( pH = 9.4) in less than 200 s. Linear calibration curves were obtained with limits of detection of 80 mM, 250 mM and 270 mM for creatinine, creatine and uric acid respectively. An optimization of the separation conditions and a comparison of PAD with other amperometric detection modes is also shown. Finally, analysis of a real urine sample is presented with validation of creatinine concentrations using a clinical assay kit based on the Jaffe reaction.