Rhizobium selenireducens sp Nov.:: A selenite-reducing α-Proteobacteria isolated from a bioreactor
Rhizobium selenireducens sp Nov.:: A selenite-reducing α-Proteobacteria isolated from a bioreactor
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DOI：
10.1007/s00284-007-9020-9
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发表时间：
2007-11-01
影响因子：
2.6
通讯作者：
Manter, D. K.
中科院分区：
文献类型：
--
作者：
Hunter, W. J.;Kuykendall, L. D.;Manter, D. K.
从实验室生物反应器中分离到一株革兰氏阴性、无色素的细菌B1，该细菌将硒酸盐还原为元素红硒（Se-0）。16 S rRNA基因序列比对鉴定该分离物为根瘤菌属（Rhizobium sp.），属于根瘤菌属（Rhizobium clade）分支，该分支包括R.大田河贾迪尼河温迪科拉河（R.拉里穆尔河拉迪沃河鲁比和R.葡萄属R. radiodenum和R.鲁比是其最近的亲戚，16 S rRNA基因序列比对表明，这是由2.6%和2.8%，分别不同于菌株B1。在这一组中，表现出方差> 0.8%至2.2%的菌株被分类为不同的种。B1菌株中存在的主要细胞脂肪酸为C16：0（1.8%）、C18：0（3.38%）、18：0 3-OH（1.6%）、18：1 omega 7 c（86.8%）、19：0环omega 8 c（1.5%）以及总特征2（3.8%）和3（1.2%）。大量的18：1 ω 7 c存在于该组细菌的成员中是恒定的，但是少量的16：0、19：0环ω 8 c和求和特征3显示出与R的差异。radiodenum和R.鲁比。该菌株的表型和生化特征与其在该属中的位置一致。
A Gram-negative, nonpigmented bacterium designated strain B1 was isolated from a laboratory bioreactor that reduced selenate to elemental red selenium (Se-0). 16S rRNA gene-sequence alignment identified the isolate as a Rhizobium sp. belonging to the Rhizobium clade, which includes R. daejeonense, R. giardinii, R. undicola, R. larrymoorei, R. radiobacter, R. rubi, and R. vitis. R. radiobacter and R. rubi are its closest relatives as indicated by 16S rRNA gene-sequence alignments, which differ from strain B1 by 2.6% and 2.8%, respectively. Within this group, strains that show variances > 0.8% to 2.2% have been classified as different species. The major cellular fatty acids present in the B1 strain were C16:0 (1.8%), C18:0 (3.38%), 18:0 3-OH (1.6%), 18:1 omega 7c (86.8%), 19:0 cyclo omega 8c (1.5%), and summed features 2 (3.8%) and 3 (1.2%). The large amount of 18:1 omega 7c present is constant with members of this group of bacteria, but the small amounts of 16:0, 19:0 cyclo omega 8c, and summed feature 3 shows variance from R. radiobacter and R. rubi. The strain's phenotypic and biochemical characteristics are consistent with its placement in this genus.