A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis
A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis
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DOI：
10.1073/pnas.0408952102
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发表时间：
2005-01-18
影响因子：
11.1
通讯作者：
Richardson, PM
中科院分区：
文献类型：
--
作者：
Keys, DN;Lee, BI;Richardson, PM
使用基础脊索动物玻璃海鞘对含有 Hox 基因的大（> 100 kb）基因组结构域内的顺式调控元件进行了系统鉴定的筛选。将含有两簇 Hox 基因的细菌人工染色体随机生成的 DNA 片段插入到最小启动子和 lacZ 报告基因上游的载体中。将总共222个融合基因分别电穿孔到受精卵中，并在幼虫中监测它们的调节活性。总之，发现了 21 个可分离的顺式调控元件。其中包括八个 Hox 连接结构域，驱动外胚层衍生组织的嵌套前-后结构域中的表达。除了类似脊椎动物的中枢神经系统调节之外，驱动表皮转录的顺式调节结构域的发现表明，肠杆菌在表皮中具有类似节肢动物的 Hox 模式。
A screen for the systematic identification of cis-regulatory elements within large (> 100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. In sum, 21 separable cis-regulatory elements were found. These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived tissues. In addition to vertebrate-like CNS regulation, the discovery of cis-regulatory domains that drive epidermal transcription suggests that C. intestinalis has arthropod-like Hox patterning in the epidermis.