STABILITY OF PYRIDOXAL-5-PHOSPHATE SEMICARBAZONE - APPLICATIONS IN PLASMA VITAMIN-B6 ANALYSIS AND POPULATION SURVEYS OF VITAMIN-B6 NUTRITIONAL-STATUS
STABILITY OF PYRIDOXAL-5-PHOSPHATE SEMICARBAZONE - APPLICATIONS IN PLASMA VITAMIN-B6 ANALYSIS AND POPULATION SURVEYS OF VITAMIN-B6 NUTRITIONAL-STATUS
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DOI：
10.1016/s0378-4347(00)84518-1
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
DEVILLIERS, LS
中科院分区：
文献类型：
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作者：
UBBINK, JB;SERFONTEIN, WJ;DEVILLIERS, LS
血浆中吡哆醛-5-磷酸（PLP）和吡哆醛（PL）的测定需要暗室设备，因为这些维生素B6维生素具有光敏性。在血浆分析中已经利用了缩氨基脲形式的PL和PLP比未活化的B6维生素具有更强的荧光性，但以前没有意识到，即使在导致游离PL和PLP快速分解的条件下，这些缩氨基脲形式也非常稳定。以这种方式获得的PLP和PL的稳定性是足够的，完全足以满足临床领域研究的实际要求。本文报道了基于柱前缩卡酮形成和荧光检测的高效液相色谱法测定血浆PLP和PL的方法。该方法对定量测定血浆PLP足够敏感，即使在b缺乏患者中也是如此。
The determination of pyridoxal-5-phosphate (PLP) and pyridoxal (PL) in plasma requires the availability of dark room facilities, due to the photosensitivity of these vitamin B6 vitamers. The fact that the semicarbazone forms of PL and PLP are more strongly fluorescent than the underivatized B6 vitamers has been exploited in plasma analyses, but it was not previously realised that these semicarbazone forms are also very stable even under conditions that lead to rapid decomposition of free PL and PLP. The stabilisation of PLP and PL obtained in this manner is sufficient and fully adequate to meet the practical requirements of clinical field studies. We report a high-performance liquid chromatographic method for plasma PLP and PL determinations based on precolumn semicarbaxone formation and fluorescence detection. The method is sensitive enough for quantitative plasma PLP determinations even in B-deficient patients.