DNA typing of human dandruff.
DNA typing of human dandruff.
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人类头皮屑的 DNA 分型。
DOI：
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发表时间：
1998
影响因子：
1.6
通讯作者：
K. Herold
中科院分区：
文献类型：
--
作者：
B. Herber;K. Herold
头皮屑颗粒在无核角质细胞的聚集体内含有相当大部分的有核细胞。由于表皮分化过程，细胞核可部分降解。为了测试头皮屑DNA在法医应用中的适用性，使用不同的STR多态性对35名受试者和两起犯罪案件进行了研究。在90%的样品中，可以容易地进行DNA基因分型，表明来自头皮屑的DNA至少适合于STR分析。每个头皮屑颗粒的DNA量对于测试对象在0.8至16.6ng DNA的范围内变化极大。如果DNA提取量减少，也可以对单个头皮屑颗粒进行基因分型。在头皮屑与血渍或精斑共提取的混合样本中，头皮屑基因型是可检测的。在某些情况下，这是一个很大的问题。这些结果表明头皮屑在法医案例工作中的相关性，无论是作为额外的样品还是作为无意污染的来源。
Dandruff particles contain a considerable portion of nucleated cells within the aggregates of nuclei-free corneocytes. The nuclei could be partially degraded due to epidermal differentiation processes. To test the suitability of DNA from dandruff for forensic application, a study of 35 subjects and two crime cases has been carried out using different STR polymorphisms. In 90% of the samples DNA genotyping could be easily performed indicating that DNA from dandruff is at least suitable for STR analysis. DNA quantity per dandruff particle varies extremely within a range from 0.8 to 16.6 ng DNA for the tested subjects. Genotyping was also possible for a single dandruff particle if DNA extraction volume had been reduced. In mixed samples from dandruff coextracted with bloodstains or semen stains dandruff genotype was detectable. in some cases. These results demonstrate the relevance of dandruff in forensic casework either as an additional sample or as a source of inadvertent contamination.
影响因子：
9.8
作者：
B. Budowle;R. Chakraborty;A. Giusti;A. Eisenberg;R. Allen
通讯作者：
B. Budowle;R. Chakraborty;A. Giusti;A. Eisenberg;R. Allen
人α纤维蛋白原基因座（FGA）的四核苷酸重复多态性。
影响因子：
3.5
作者：
Mills,KA;Even,D;Murray,JC
通讯作者：
Murray,JC