α-Pinene, linalool, and 1-octanol contribute to the topical anti-inflammatory and analgesic activities of frankincense by inhibiting COX-2
α-Pinene, linalool, and 1-octanol contribute to the topical anti-inflammatory and analgesic activities of frankincense by inhibiting COX-2
复制标题
α-蒎烯、芳樟醇和 1-辛醇通过抑制 COX-2 促进乳香的局部抗炎和镇痛活性
DOI：
10.1016/j.jep.2015.12.039
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发表时间：
2016-02-17
影响因子：
5.4
通讯作者：
Tang, He-Bin
中科院分区：
文献类型：
--
作者：
Li, Xiao-Jun;Yang, Yan-Jing;Tang, He-Bin
民族药理学相关性：乳香油和水提取物（FOE，FWE）长期以来一直用于炎症和疼痛的外部治疗。本研究的目的是鉴定负责抗炎和镇痛作用的活性成分，并确定潜在的机制。材料和方法：采用GC-MS法对两种提取物的化学成分进行了鉴定和比较，并对两种提取物的抗炎镇痛活性及其可能的活性成分进行了研究在二甲苯诱导的耳水肿模型和福尔马林发炎的后爪模型中评价和比较α-蒎烯、芳樟醇和1-辛醇的含量。结果：FOE中α-蒎烯、芳樟醇和正辛醇的含量明显高于FWE，而FWE中α-蒎烯、芳樟醇和正辛醇的含量明显高于FWE。用FOE治疗的小鼠比用FWE治疗的小鼠表现出更大和更快的肿胀和疼痛减轻。这三种成分的组合有更强的药理作用，对后爪炎症和考克斯-2过表达比单独使用的三个组件。结论：这些研究结果表明，局部应用FOE或其活性成分（包括α-蒎烯，芳樟醇，1-辛醇）表现出显着的抗炎和镇痛作用，通过抑制伤害性刺激诱导的炎症浸润和考克斯-2过表达。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Frankincense oil and water extracts (FOE, FWE) have long been used for external treatment of inflammation and pain. The present study was conducted to identify the active ingredients responsible for the anti-inflammatory and analgesic effects and to determine the underlying mechanisms.Materials and methods: The compositions of FOE and FWE were identified and compared by GC-MS. The anti-inflammatory and analgesic activities of the two extracts and their possible active ingredients (alpha-pinene, linalool, and 1-octanol) were evaluated and compared in a xylene-induced ear edema model and a formalin-inflamed hind paw model. Inflammatory infiltrates and cyclooxygenase-2 (COX-2) expression in hind paw skin were investigated by histological staining.Results: The contents of alpha-pinene, linalool, and 1-octanol in FOE were much higher than those in FWE. Mice treated with FOE exhibited greater and faster lessening of swelling and pain than mice treated with FWE. The combination of the three components had more potent pharmacological effects on hind paw inflammation and COX-2 overexpression than the three components used alone.Conclusions: These findings suggest that topical application of FOE or its active ingredients (including alpha-pinene, linalool, and 1-octanol) exhibit significantly anti-inflammatory and analgesic effects through inhibiting nociceptive stimulus-induced inflammatory infiltrates and COX-2 overexpression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.