Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR
Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR
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DOI：
10.1089/mdr.2008.0772
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Wang, Honghai
中科院分区：
文献类型：
--
作者：
Jiang, Xin;Zhang, Wenhong;Wang, Honghai
通过使用具有 rpoB 和 katG 确定突变的多重耐药 (MDR) 菌株 1499，确定了一些推定的外排泵在赋予结核分枝杆菌某些菌株耐药性方面的重要性。通过实时 RT-PCR 测试了临床分离株 1499 的药物敏感性和 5 个假定的外排泵基因的表达水平。在利福平或异烟肼存在的情况下，该分离株显示推定的外排泵基因 Rv1258c 和 Rv1410c 的转录增加。 Rv1819c 仅在异烟肼暴露时过度表达 (p
The importance of some putative efflux pumps in conferring drug resistance on certain strain of Mycobacterium tuberculosis was determined by using multidrug-resistant (MDR) strain 1499 bearing defined mutations in rpoB and katG. The clinical isolate 1499 was tested for drug sensitivity and expression level of five putative efflux pump genes by real-time RT-PCR. In the presence of rifampicin or isoniazid, this isolate showed increased transcription of putative efflux pump genes Rv1258c and Rv1410c. Rv1819c was overexpressed only upon isoniazid exposure (p