Focusing of low-molecular-mass heparins in polycationic polyacrylamide matrices.
Focusing of low-molecular-mass heparins in polycationic polyacrylamide matrices.
复制标题
低分子量肝素在聚阳离子聚丙烯酰胺基质中的聚焦。
DOI：
10.1021/ac901050q
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发表时间：
2009
影响因子：
7.4
通讯作者：
Bukshpan,Shmuel
中科院分区：
文献类型：
--
作者：
Zilberstein,Gleb;Shlar,Ilya;Korol,Leonid;Baskin,Emmanuil;Fasoli,Elisa;Righetti,PierGiorgio;Torri,Giangiacomo;Bisio,Antonella;Bukshpan,Shmuel
A novel method for separation of low-molecular-mass heparins is reported here, on the basis of migrating the polyanionic heparins in a polycationic polyacrylamide gel, made by incorporating a gradient of positively charged monomers (the Immobilines used for creating immobilized pH gradients) into the neutral polyacrylamide backbone. Separations can be operated either in linear or nonlinear gradients of positive charges, thus modulating at whim the separation power. This allows the polydisperse heparins to reach a steady-state position along the migration path and condense (focus) in an environment inducing charge neutralization. It is shown that the separations obtained are a complex function of both size and charge distribution along the oligosaccharide chains. This novel methodology represents a marked improvement over existing techniques and appears to hold promise for applications in screening of commercial lots of heparins, also in view of possible presence of contaminants, such as those recently detected in imported heparins.
DOI：
--
发表时间：
1982
期刊：
影响因子：
--
作者：
D. Rickwood;B. Hames
通讯作者：
B. Hames
固定化 Ph 梯度：理论与方法
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
P. Righetti
通讯作者：
P. Righetti