FcRY an Fc receptor related gene differentially expressed during B lymphocyte development and activation
FcRY an Fc receptor related gene differentially expressed during B lymphocyte development and activation
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DOI：
10.1016/j.gene.2005.08.016
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发表时间：
2005-12-19
期刊：
影响因子：
3.5
通讯作者：
Burrows, PD
中科院分区：
文献类型：
--
作者：
Masuda, K;Davis, RS;Burrows, PD
生物信息学方法已经鉴定了一个新的Fc受体相关基因FcRY。预测FcRY编码具有三个免疫球蛋白（IG）结构域的蛋白质，随后是含有富含脯氨酸的茎和C-末端富含亮氨酸的区域的粘蛋白样结构域。预测的蛋白质缺乏插入质膜的疏水结构域，表明FcRY是细胞内或分泌蛋白。该特征与FcRX/FCRL/FREB基因的产物共享，所述FcRX/FCRL/FREB基因在人和小鼠基因组中均与FcRY紧密连锁。在前B细胞阶段的小鼠B谱系细胞中首先可检测到Fcry转录物。新形成的脾B细胞、滤泡和边缘区亚群表达Fc γ，生殖中心B细胞表达程度较低。FcRY也在人B细胞亚群中表达。FcRY在两个物种中的一致特征是低水平基因表达，其可通过经由B细胞受体（BCR）或CD 40的信号传导在正常小鼠B细胞中进一步下调，从而表明细胞周期进入和减少的FcRY表达之间的相关性。用BAFF/BLyS短期处理可上调Fcry，其促进B细胞存活而非增殖。LPS诱导非常快速但短暂的增强。我们观察到BCR交联诱导WEHI 231细胞中的Fcry表达显著上调，从而在凋亡之前经历细胞周期停滞，这与细胞周期状态对Fcry表达的可能调节一致。(c)2005 Elsevier B. V.保留所有权利。
A bioinformatics approach has lead to the identification of FcRY, a new Fc receptor related gene. FcRY is predicted to encode a protein with three immunoglobulin (Ig) domains followed by a mucin-like domain containing a proline-rich stalk and a C-terminal leucine rich region. The predicted protein lacks a hydrophobic domain for insertion into the plasma membrane, suggesting that FcRY is an intracellular or secreted protein. This feature is shared with the product of the FcRX/FCRL/FREB gene that is closely linked to FcRY in both human and mouse genomes. Fcry transcripts are first detectable among mouse B lineage cells at the pre-B cell stage. Splenic B cells of the newly formed, follicular, and marginal zone subsets express Fcry, as do germinal center B cells to a lesser extent. FcRY is also expressed in subpopulations of human B cells. A consistent characteristic of FcRY in both species is low level gene expression, which can be further downregulated in normal mouse B cells by signaling through the B cell receptor (BCR) or CD40, thereby suggesting a correlation between cell cycle entrance and diminished FcRY expression. Fcry is upregulated by short-term treatment with BAFF/BLyS, which promotes B cell survival rather than proliferation. LPS induces very rapid but transient enhancement. We observed a pronounced upregulation of Fcry expression in WEHI 231 cells induced by BCR crosslinking to undergo cell cycle arrest prior to apoptosis, consistent with the possible regulation of Fcry expression by cell cycle status. (c) 2005 Elsevier B.V. All rights reserved.