Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
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DOI：
10.1007/978-1-62703-487-6_1
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Bruegger, Britta
中科院分区：
文献类型：
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作者：
Oezbalci, Cagakan;Sachsenheimer, Timo;Bruegger, Britta
通过纳米电喷雾电离质谱法进行的脂质分析是一种高度敏感的脂质定量方法，包括给定脂类的所有脂质种类。各种仪器设置用于定量脂质分析，包括不同模式的电离，分离和检测。在这里，我们描述了一种快速定量分析细胞膜脂质的工作流程，通过将脂质提取物直接注入纳米电喷雾电离三重四极杆/线性离子阱质谱仪。
Lipid analysis performed by nano-electrospray ionization mass spectrometry is a highly sensitive method for quantification of lipids including all lipid species of a given lipid class. Various instrumental setups are used for quantitative lipid analysis, including different modes of ionization, separation, and detection. Here we describe a work-flow for the rapid and quantitative analysis of lipid species from cellular membranes by direct infusion of lipid extracts to a nano-electrospray ionization triple quadrupole/linear ion trap mass spectrometer.