Activity Patterns of Inhibitory Motoneurones and their Impact on Leg Movement in Tethered Walking Locusts
Activity Patterns of Inhibitory Motoneurones and their Impact on Leg Movement in Tethered Walking Locusts
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束缚行走蝗虫抑制性运动神经元的活动模式及其对腿部运动的影响
DOI：
10.18725/oparu-1249
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
H. Wolf
中科院分区：
文献类型：
--
作者：
H. Wolf
本文描述了一种能在细胞内记录拴系步行蝗虫神经活动的制剂。需要进行最小程度的解剖，使动物几乎完好无损。在这个准备，活动的三个共同的抑制性运动神经元（CI）供应的中腿的肌肉被记录。CI是完全或几乎完全沉默的静态蝗虫，但经常产生紧张尖峰放电在步行活动。这种紧张性放电在步周期中被调制，动作电位的爆发与腿部运动的摆动相结合而产生。爆发在腿前伸开始时达到峰值。他们在所有三个CI和各种不同的步行情况下（向前和向后步行，搜索等）非常相似。唯一值得注意的区别是，CI 1的爆发通常比CI 2的爆发早40 ms达到峰值。这些结果表明，CI活动的时间根据受神经支配的肌肉群，但没有进一步的功能专业化存在。通过电流注入操纵Cl 1的活性。例如，通过施加超极化电流来减少CI尖峰放电会降低行走动物的腿前伸速度。这表明CI 1在确定挥杆运动的速度方面起着重要作用。
A preparation is described which permits intracellular recording of neural activity in tethered walking locusts. Minimal dissection is required, leaving the animal nearly intact. In this preparation, the activities of the three common inhibitory motoneurones (CIs) supplying the muscles of the middle leg were recorded. CIs were completely or almost completely silent in quiescent locusts but often produced a tonic spike discharge during walking activity. This tonic discharge was modulated in the step cycle, bursts of action potentials being generated in conjunction with the swing phase of the leg movement. The bursts peaked at around the start of leg protraction. They were remarkably similar in all three CIs and in a variety of different walking situations (forward and backward walking, searching, etc.). The only notable difference was that bursts of CI 1 normally peaked some 40 ms before those of CI 2 . These results indicate that CI activity is timed according to the muscle group innervated but that no further functional specializations exist. Activity of CI 1 was manipulated by current injection. For example, decreasing CI spike discharge by the application of hyperpolarizing current reduced the velocity of leg protraction in the walking animal. This demonstrates that CI 1 plays an important role in determining the speed of the swing movement.