DNA Contents of Cell Nuclei in Two Cyanidiophyceae: Cyanidioschyzon merolae and Cyanidium caldarium Forma A
DNA Contents of Cell Nuclei in Two Cyanidiophyceae: Cyanidioschyzon merolae and Cyanidium caldarium Forma A
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两种蓝藻纲：Cyanidioschyzon merolae 和 Cyanidium caldarium Forma A 细胞核的 DNA 含量
DOI：
10.1508/cytologia.60.183
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发表时间：
1995
期刊：
影响因子：
1
通讯作者：
T. Kuroiwa
中科院分区：
文献类型：
--
作者：
K. Toda;Hidenori Takahashi;R. Itoh;T. Kuroiwa
对蓝藻C. merolae和C.用DAPI、Schiff's试剂或PI染色后，使用VIMPCS估计caldarium Forma A。梭并与脉冲场凝胶电泳的结果进行了比较。结果表明，C. merolae和C. caldarium Forma A的分子量分别约为13 Mbp和35 Mbp。由于PFGE获得的值非常接近从Feulgen染色估计的值，结合嘌呤残基且不受GC含量影响的Schiff试剂被认为是这三种试剂中用于估计DNA含量的最可靠的试剂。
The DNA contents in the cell nuclei of the Cyanidiophyceae C. merolae and C. caldarium Forma A were estimated using VIMPCS after staining with DAPI, Schiff's reagent or PI. For C. merolae, the obtained data were compared with those of PFGE. The results indicated that the genome sizes of C. merolae and C. caldarium Forma A were approximately 13 Mbp and 35 Mbp, respectively. Since the values obtained by PFGE were very close to those estimated from Feulgen staining, Schiff's reagent, which binds to purine residues and is not affected by GC content, is considered to be the most reliable of these three reagents for estimating DNA contents.