Differential gene expression profile in the liver of the marine puffer fish Takifugu rubripes induced by intramuscular administration of tetrodotoxin
Differential gene expression profile in the liver of the marine puffer fish Takifugu rubripes induced by intramuscular administration of tetrodotoxin
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DOI：
10.1016/j.toxicon.2010.12.007
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发表时间：
2011-02-01
期刊：
影响因子：
2.8
通讯作者：
Nagashima, Yuji
中科院分区：
文献类型：
--
作者：
Matsumoto, Takuya;Ishizaki, Shoichiro;Nagashima, Yuji
河豚鱼在肝脏和卵巢中积累了大量的河豚毒素，但河豚毒素积累的机制尚不清楚。为了阐明河豚鱼肝脏变性相关基因，我们采用抑制性消减杂交技术研究了红鳍东方鲀（Takifugu rubripes）尾肌肌肉注射0.50 mg/kg TTX后肝脏基因的表达谱。在给药后12小时内，TTX在肝脏中的蓄积达到给药剂量的68 +/- 4%。从消减cDNA文库中成功测序了1048个克隆。1048个克隆中92个的核苷酸序列被鉴定为铁调素前体。逆转录-聚合酶链反应实验表明，hepcidin前体在TTX给药组中高度表达。另外，获得补体C3（31个克隆）、血清转铁蛋白（30个克隆）、载脂蛋白A-1（14个克隆）、高温适应蛋白Wap 65 -2（14个克隆）、补体C7（12个克隆）、纤维蛋白原β链（12个克隆）和70 kDa热休克蛋白4（11个克隆）。这项研究证实，肌肉注射TTX增加急性期反应蛋白的基因表达的河豚鱼T rubripes的肝脏。(c)2010年由Elsevier Ltd.出版
Marine puffer fish accumulate a high level of tetrodotoxin (TTX) in the liver and ovary, but the underlying mechanism of this toxification is unclear. To elucidate the genes related to toxification of the marine puffer fish, we examined the hepatic gene expression profile of the marine puffer fish Takifugu rubripes by suppression subtractive hybridization in response to the intramuscular administration of 0.50 mg TTX/kg body weight into the caudal muscle. The accumulation of TTX in the liver reached 68 +/- 4% that of the administered dose within 12 h of administration. A total of 1048 clones from the subtracted cDNA libraries were successfully sequenced. The nucleotide sequence of 92 of the 1048 clones was identified as a hepcidin precursor. Reverse transcription-polymerase chain reaction experiments revealed that hepcidin precursors were highly expressed in the TTX-administered group. In addition, complement C3 (31 clones), serotransferrin (30 clones), apolipoprotein A-1 (14 clones), high temperature adaptation protein Wap65-2 (14 clones), complement C7 (12 clones), fibrinogen beta chain (12 clones), and 70 kDa heat-shock protein 4 (11 clones) were obtained. This study confirmed that the intramuscular administration of TTX increases the gene expression of the acute-phase response proteins in the liver of puffer fish T rubripes. (c) 2010 Published by Elsevier Ltd.