Very high speed separation of proteins with a 20-μm reversed-phase sorbent
Very high speed separation of proteins with a 20-μm reversed-phase sorbent
复制标题
DOI：
10.1016/s0021-9673(01)88671-4
复制
发表时间：
1991-06
影响因子：
4.1
通讯作者：
S. Fulton;N. Afeyan;N. Gordon;F. Regnier
中科院分区：
文献类型：
--
作者：
S. Fulton;N. Afeyan;N. Gordon;F. Regnier
在20 μm聚合物流通型色谱填料（波罗斯R/M）上，通过反相梯度洗脱，在15 s内成功分离了5种蛋白质混合物。通过简单地扩展梯度体积，在同一柱上也可以在小于60秒内以更好的分辨率分离相同的混合物。尽管表观线速度接近9000 cm/h，但穿过柱的压降小于130 bar。使用不同流速下的前沿色谱法来证明高速分离的能力是由于流过（灌注色谱法）效应导致的颗粒内极大增强的质量传递。
A five-protein mixture was successfully separated in less than 15 s by reversed-phase gradient elution on a 20-μm polymeric flow-through type chromatographic packing material (POROS R/M). The same mixture could also be separated with far better resolution in less than 60 s on the same column by simply extending the gradient volume. Pressure drop across the column was less than 130 bar, despite a superficial linear velocity of nearly 9000 cm/h. Frontal chromatography at different flow-rates was used to demonstrate that the ability to separate at high speed was due to greatly enhanced mass transport within the particles due to the flow-through (perfusion chromatography) effect.