Radiation Induces Phosphorylation of STAT3 in a Dose- and Time-dependent Manner
Radiation Induces Phosphorylation of STAT3 in a Dose- and Time-dependent Manner
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辐射以剂量和时间依赖性方式诱导 STAT3 磷酸化。
DOI：
10.7314/apjcp.2014.15.15.6161
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Su, Xu
中科院分区：
文献类型：
--
作者：
Gao, Ling;Li, Feng-Sheng;Su, Xu
背景：我们已报道辐射可激活STAT3，从而促进A549细胞的侵袭。我们在这里探索了STAT3对辐射的剂量和时间响应以及辐射对上游信号分子的影响。材料和方法：用不同剂量的伽玛射线照射A549细胞。免疫印迹法检测p-STAT3在A549细胞中的表达，免疫荧光法检测p-STAT3的核转位。免疫印迹法检测EGFR的磷酸化水平，实时荧光定量聚合酶链式反应和酶联免疫吸附试验检测IL-6的表达。结果：辐射以剂量和时间依赖的方式促进Y705位点STAT3的磷酸化和核转位。照射后A549细胞的磷酸化EGFR水平升高。此外，辐射还上调了A549细胞IL-6的mRNA和蛋白水平。结论：辐射对A549细胞STAT3的激活具有剂量和时间依赖性，可能与辐射诱导A549细胞EGFR活化或分泌IL-6有关。
Background: We have reported the radiation could activate STAT3, which subsequently promotes the invasion of A549 cells. We here explored the dose-and time-response of STAT3 to radiation and the effect of radiation on upstream signaling molecules. Materials and Methods: A549 cells were irradiated with different doses of gamma-rays. The expression of and nucleus translocation of p-STAT3 in A549 cells were detected by immunoblotting and immunofluorescence, respectively. The level of phosphorylated EGFR was also assessed by immunoblotting, and IL-6 expression was detected by real time PCR and ELISA. Results: Radiation promoted the phosphorylation of STAT3 at Y705 in a dose-and time-dependent manner and nuclear translocation. The level of phosphorylated EGFR in A549 cells increased after radiation. In additional, the mRNA and protein levels of IL-6 in A549 cells were also up regulated by radiation. Conclusions: STAT3 is activated by radiation in a dose-and time-dependent manner, probably due to radiation-induced activation of EGFR or secretion of IL-6 in A549 cells.