Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea).
Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea).
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精细作图和转录组分析揭示了与卷心菜（Brassica Oleracea）杂交致死性相关的候选基因
DOI：
10.3390/genes8060147
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发表时间：
2017-06-05
期刊：
影响因子：
3.5
通讯作者：
Zhuang M
中科院分区：
文献类型：
--
作者：
Xiao Z;Hu Y;Zhang X;Xue Y;Fang Z;Yang L;Zhang Y;Liu Y;Li Z;Liu X;Liu Z;Lv H;Zhuang M
杂交致死性是一种对物种进化至关重要的有害表型。我们之前报道了卷心菜（Brassica oleracea）的杂交致死性，并对相关基因进行了初步定位。本研究通过对杂交致死基因的精细定位发现，BoHL1位于C1染色体上BoHLTO124和BoHLTO130之间，间隔101 kb。 BoHL2被证实位于C4上插入缺失(InDels)标记HL234和HL235之间，标记间隔为70kb。在 BoHL1 和 BoHL2 的两个区间内分别发现了 28 个和 9 个注释基因。我们还应用 RNA-Seq 来分析卷心菜的杂交致死率。在BoHL1区域中，发现了7个差异表达基因（DEG）和5个抗性（R）相关基因（两个共同点，即Bo1g153320和Bo1g153380），而在BoHL2区域中，发现了2个DEG和4个R相关基因（两个共同点，即Bo4g173780和Bo4g173810）。连同 R 基因经常参与其他植物杂交致死性的研究一起，这些有趣的 R-DEG 可能是与杂交致死性相关的良好候选者。我们还使用 SNP/InDel 分析和定量实时 PCR 来确认结果。这项工作为卷心菜的混合致死机制提供了新的见解。
Hybrid lethality is a deleterious phenotype that is vital to species evolution. We previously reported hybrid lethality in cabbage (Brassica oleracea) and performed preliminary mapping of related genes. In the present study, the fine mapping of hybrid lethal genes revealed that BoHL1 was located on chromosome C1 between BoHLTO124 and BoHLTO130, with an interval of 101 kb. BoHL2 was confirmed to be between insertion-deletion (InDels) markers HL234 and HL235 on C4, with a marker interval of 70 kb. Twenty-eight and nine annotated genes were found within the two intervals of BoHL1 and BoHL2, respectively. We also applied RNA-Seq to analyze hybrid lethality in cabbage. In the region of BoHL1, seven differentially expressed genes (DEGs) and five resistance (R)-related genes (two in common, i.e., Bo1g153320 and Bo1g153380) were found, whereas in the region of BoHL2, two DEGs and four R-related genes (two in common, i.e., Bo4g173780 and Bo4g173810) were found. Along with studies in which R genes were frequently involved in hybrid lethality in other plants, these interesting R-DEGs may be good candidates associated with hybrid lethality. We also used SNP/InDel analyses and quantitative real-time PCR to confirm the results. This work provides new insight into the mechanisms of hybrid lethality in cabbage.