Effects of thyrotrophin stimulation for two hours on mouse thyroid cyclic AMP levels in vivo and in vitro.
Effects of thyrotrophin stimulation for two hours on mouse thyroid cyclic AMP levels in vivo and in vitro.
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DOI：
10.1016/0024-3205(82)90732-9
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发表时间：
1982-12
期刊：
影响因子：
6.1
通讯作者：
B. Ahrén;A. Gustafson;P. Hedner
中科院分区：
文献类型：
--
作者：
B. Ahrén;A. Gustafson;P. Hedner
促甲状腺素（TSH）刺激小鼠体内2小时，反映了血浆三碘甲腺原氨酸（T3）水平的持续增加，与甲状腺环磷酸腺苷（cAMP）含量的增加，在第一个25分钟的刺激1，此后的水平迅速下降。在体外ISH刺激小鼠甲状腺组织2h时，发现了类似的cAMP反应模式。这是一种cAMP对长期激素刺激的体内反应，在包括甲状腺组织在内的几种体外系统中观察到，通常称为激素诱导的腺苷酸环化酶脱敏。目前的结果表明，这种现象不仅限于在体外条件下，但也可以证明在体内，并支持在这方面的体外实验的代表性。
A thyrotrophin (TSH) stimulation in vivo in mice for 2 h, reflected by continously increasing plasma triiodothyronine (T3) levels, was associated with an increase in the thyroid content of cyclic AMP (cAMP) during the first 25 min of stimulationl thereafter the level rapidly declined. A similar pattern of the cAMP response was found when mouse thyroid tissue was stimulated by ISH in vitro for 2 h. This is an in vivo demonstration of a type of cAMP response to prolonged hormonal stimulation that has been observed in several in vitro systems including thyroid tissue, generally referred to as hormone induced desensitization of adenyl cyclase. The present results indicate that the phenomenon is not confined to in vitro conditions but can be demonstrated also in vivo, and support the representativeness of in vitro experiments in this respect.