Immunohistochemical expression of nuclear and cytoplasmic survivin in gastrointestinal carcinoma.
Immunohistochemical expression of nuclear and cytoplasmic survivin in gastrointestinal carcinoma.
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DOI：
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发表时间：
2013-11
影响因子：
1.4
通讯作者：
M. Shintani;Akiko Sangawa;N. Yamao;S. Kamoshida
中科院分区：
文献类型：
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作者：
M. Shintani;Akiko Sangawa;N. Yamao;S. Kamoshida
存活素是一种在许多胚胎组织和大多数人类肿瘤中高度表达的蛋白质。先前的研究报道了survivin表达与癌症预后之间的正相关和负相关，但这些关联仍然存在争议。在本研究中，我们评估了核和细胞质survivin在胃肠道癌中的表达。利用这些数据，我们确定了细胞核和细胞质survivin之间的相关性，并进一步研究了survivin表达与临床病理参数之间的相关性。应用免疫组化方法分析72例晚期胃腺癌和78例结直肠腺癌的survivin表达情况。核survivin和胞质survivin在结直肠癌中的表达均显著高于胃癌（P < 0.01）。survivin的核表达与细胞质表达呈正相关（r = 0.42, P < 0.001）。在胃癌中，survivin蛋白表达水平与肿瘤分化、患者年龄、淋巴浸润相关（P分别< 0.05、0.01、0.01）。在结直肠癌中，核survivin在女性中的表达水平显著高于男性（P < 0.05）。survivin表达与大多数临床病理参数之间无显著相关性。然而，在胃癌中，核survivin的表达与肿瘤侵袭性呈负相关；细胞质survivin的表达也有类似的趋势。总之，我们的研究结果表明，胃癌和结直肠癌的细胞核和细胞质survivin表达水平不同。
Survivin is a protein that is highly expressed in many embryonic tissues, as well as most human tumors. Prior studies have reported both positive and negative correlations between survivin expression and cancer prognosis, but these associations remain controversial. In the present study, we assessed the expression of nuclear and cytoplasmic survivin in gastrointestinal carcinomas. Using these data, we determined the correlation between nuclear and cytoplasmic survivin and, further, investigated correlations between survivin expression and clinicopathological parameters. Seventy-two advanced gastric adenocarcinomas and 78 colorectal adenocarcinomas were analyzed for survivin expression by immunohistochemistry. Expression of both nuclear and cytoplasmic survivin was significantly higher in colorectal carcinomas than in gastric carcinomas (P < 0.01). There was a positive correlation between nuclear and cytoplasmic expression of survivin (r = 0.42, P < 0.001). In gastric carcinomas, the level of survivin protein expression was associated with tumor differentiation, patient age, and lymphatic invasion (P < 0.05, 0.01, and 0.01, respectively). In colorectal carcinomas, the level of nuclear survivin expression was significantly higher in females than in males (P < 0.05). There were no significant associations between survivin expression and most of the clinicopathological parameters. Nevertheless, there was a trend towards an inverse correlation between nuclear survivin expression and tumor aggressiveness in gastric carcinoma; there was a similar trend for cytoplasmic survivin expression. In summary, our results suggest that levels of nuclear and cytoplasmic survivin expression differ between gastric carcinoma and colorectal carcinoma.