Relationship between in vitro tumor stem cell assay and in vivo antitumor activity using the P388 leukemia.
Relationship between in vitro tumor stem cell assay and in vivo antitumor activity using the P388 leukemia.
复制标题
使用 P388 白血病进行体外肿瘤干细胞测定与体内抗肿瘤活性之间的关系。
DOI：
10.1002/stem.5530060107
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Venditti,JM
中科院分区：
文献类型：
--
作者：
Marsh,JC;Shoemaker,RH;Salmon,SE;Kern,DH;Venditti,JM
采用腹腔内P388小鼠白血病系统，研究了13种活性化疗药物的体外肿瘤干细胞敏感性与体内抗肿瘤疗效之间的关系。给药1天、5天和9天后寿命延长50%的剂量均与7天连续药物暴露期间菌落形成减少70%的体外浓度显著相关。5天的相关性最好，其次是9天和1天。通过校正体外药物稳定性或毒性（LD 50），相关性未得到改善。在这种简单的回顾性分析中观察到的高度显著的相关性为开发更复杂的模型提供了基础，用于从体外数据预测体内结果。
The relationship between in vitro tumor stem cell sensitivity and in vivo antitumor efficacy using 13 active antineoplastic agents was examined by means of the intraperitoneal P388 mouse leukemia system. The doses, which produced a 50% increase in life span after one, five and nine days of treatment, were all significantly correlated with the in vitro concentration producing a 70% reduction in colony formation during a seven-day continuous drug exposure. The five-day correlation was the best, followed by nine days and one day. Correlations were not improved by corrections for either in vitro drug stability or toxicity (LD50). The highly significant correlations observed in this simple retrospective analysis provide a basis for the development of more sophisticated models for the prediction of in vivo results from in vitro data.