Inducible gene targeting in mice using the Cre/lox system
Inducible gene targeting in mice using the Cre/lox system
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DOI：
10.1006/meth.1998.0593
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发表时间：
1998-04-01
期刊：
影响因子：
4.8
通讯作者：
Sauer, B
中科院分区：
文献类型：
--
作者：
Sauer, B
分子技术现在允许在小鼠中设计精确的遗传修饰。不仅可以将确定的核苷酸变化工程化到小鼠的基因组中，而且可以设计遗传开关以在任何确定的时间将任何基因（其基本分子信息可用）的表达或消融靶向任何组织。这些策略有望大大有助于增加对个体基因在发育和发病机制中的功能的理解。噬菌体P1的Cre位点特异性DNA重组酶是一种强有力的工具，既可以设计这种基因开关，也可以加速基因修饰动物的创造。精确的DNA重排和基因开关可以使用Cre重组酶以直接的方式有效地产生。结合用于控制Cre表达和功能的诱导系统，这些基于重组的策略可能对发育生物学和产生有用的人类疾病动物模型产生深远的影响。(C)北京：科学出版社.
Molecular techniques now allow the design of precise genetic modifications in the mouse. Not only can defined nucleotide changes be engineered into the genome of the mouse, but genetic switches can be designed to target expression or ablation of any gene (for which basic molecular information is available) to any tissue at any defined time. These strategies promise to contribute substantially to an increased understanding of individual gene function in development and pathogenesis. A powerful tool, both for the design of such genetic switches and for speeding the creation of gene-modified animals, is the Cre site-specific DNA recombinase of bacteriophage P1. Precise DNA rearrangements and genetic switches can be efficiently generated in a straightforward manner using Cre recombinase. In conjunction with inducible systems for controlling Cre expression and function, these recombination-based strategies are likely to have a profound impact on developmental biology and the generation of useful animal models of human disease. (C) 1998 Academic Press.