Cell type-specific markers for human glial and neuronal cells in culture.
Cell type-specific markers for human glial and neuronal cells in culture.
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培养物中人类神经胶质细胞和神经元细胞的细胞类型特异性标记。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
M. Raff
中科院分区：
文献类型：
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作者：
P. Kennedy;R. Lisak;M. Raff
我们使用细胞类型特异性标记来识别和研究 15 至 21 周大人类胎儿的视神经、脊髓和背根神经节分离细胞培养物中的主要神经细胞类别。星形胶质细胞通过细胞内神经胶质原纤维酸性蛋白的表达来识别，少突胶质细胞通过细胞表面半乳脑苷脂的表达来识别，背根神经节神经元通过其表面结合破伤风毒素的能力来识别，巨噬细胞（包括小胶质细胞）通过细胞表面Fc受体及其吞噬特性来识别。在没有神经元的情况下，少突胶质细胞在培养中持续表达半乳脑苷脂（其中一些还有髓磷脂碱性蛋白）长达 1 周。虽然一些背根神经节雪旺细胞最初在其表面表达半乳脑苷脂，并在细胞内表达髓磷脂碱性蛋白和主要外周髓磷脂糖蛋白（PO），但在培养几天后，它们不再表达可检测量的任何这些髓磷脂特异性分子。 Thy-1 样糖蛋白存在于成纤维细胞、背根神经节神经元和一些星形胶质细胞的表面，但未发现少突胶质细胞或大多数软脑膜细胞。纤连蛋白仅由成纤维细胞和软脑膜细胞表达。
We have used cell type-specific markers to identify and study the major classes of neural cells in dissociated cell cultures of optic nerve, spinal cord, and dorsal root ganglion from 15- to 21-week-old human fetuses. Astrocytes were identified by the intracellular expression of glial fibrillary acidic protein, oligodendrocytes by the cell surface expression of galactocerebroside, dorsal root ganglion neurons by their ability to bind tetanus toxin at their surface, and macrophages (including microglia) by cell surface Fc receptors and their phagocytic properties. Oligodendrocytes continued to express galactocerebroside (and some of them, also myelin basic protein) for up to 1 week in culture in the absence of neurons. While some dorsal root ganglion Schwann cells initially expressed galactocerebroside on their surface, and myelin basic protein and the major peripheral myelin glycoprotein (PO) intracellularly, they no longer expressed detectable amounts of any of these myelin-specific molecules after several days in culture. The Thy-1-like glycoprotein was found on the surface of fibroblasts, dorsal root ganglion neurons, and some astrocytes but not oligodendrocytes or the majority of leptomeningeal cells. Fibronectin was only expressed by fibroblasts and leptomeningeal cells.