Distribution of Genes Encoding Four Pathogenicity Islands (VPaIs), T6SS, Biofilm, and Type I Pilus in Food and Clinical Strains of Vibrio parahaemolyticus in China
Distribution of Genes Encoding Four Pathogenicity Islands (VPaIs), T6SS, Biofilm, and Type I Pilus in Food and Clinical Strains of Vibrio parahaemolyticus in China
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DOI：
10.1089/fpd.2009.0441
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发表时间：
2010-06-01
影响因子：
2.8
通讯作者：
Qian, Xiaoqin
中科院分区：
文献类型：
--
作者：
Chao, Guoxiang;Jiao, Xinan;Qian, Xiaoqin
副溶血性弧菌是中国等国食源性胃肠炎的主要病原。大流行的O_3：K_6克隆含有耐热的直接溶血素基因和oxRS/new基因，是1995年后食源性暴发的主要原因。以往的研究表明，致病岛1(VpaI-1)和VpaI-5的基因仅由大流行株携带，而VpaI-7和III型分泌系统2的基因与副溶血性弧菌TdH阳性菌株密切相关。本研究检测了71株食源性副溶血性弧菌和116株临床分离株的VpaI-2、VpaI-3、VpaI-4、VpaI-6、VI型分泌系统(T6SS)、生物被膜和I型菌毛基因的分布。结果表明，大多数副溶血性弧菌流行株都含有VpaI-2、T6SS和I型菌毛的完整基因。相反，大多数致病株(含tdh基因或tdh相关溶血素基因)和非致病株(不含tdh基因和trh基因)均含有VpaI-2、T6SS和I型菌毛的部分基因。VPaI-4基因仅存在于大流行毒株中。VPaI-3基因存在于大多数流行毒株和一小部分非致病毒株，主要是O_3：K_6毒株。几乎所有的菌株都携带VpaI-6和生物膜相关基因，无论其流行、致病或非致病特性如何。
Vibrio parahaemolyticus is a major cause of foodborne gastroenteritis in China, Japan, and other countries. The pandemic O3:K6 clone, which harbors thermostable direct hemolysin [tdh] gene and toxRS/new gene, is mainly responsible for the foodborne outbreaks after 1995. Previous studies showed that genes in the pathogenicity island-1 (VPaI-1) and VPaI-5 are harbored only by pandemic strains, whereas genes in VPaI-7 and type III secretion system 2 are closely associated with tdh-positive strains of V. parahaemolyticus. In this study, we examined the distribution of genes encoding VPaI-2, VPaI-3, VPaI-4, VPaI-6, type VI secretion systems (T6SS), biofilm, and type I pilus in 71 food and 116 clinical strains of V. parahaemolyticus. The results showed that most of the pandemic strains of V. parahaemolyticus harbored the complete genes of VPaI-2, T6SS, and type I pilus. In contrast, most of the pathogenic strains (harboring tdh gene or TDH-related hemolysin [trh] gene) and nonpathogenic strains (harboring neither tdh gene nor trh gene) contained partial genes of VPaI-2, T6SS, and type I pilus. Genes of VPaI-4 were exclusively present in the pandemic strains. Genes of VPaI-3 were present in most of the pandemic strains and a small percentage of nonpathogenic strains, mainly O3:K6 strains. VPaI-6 and biofilm-associated genes were harbored by almost all the strains, irrespective of their pandemic, pathogenic, or nonpathogenic traits.