Posttrapscriptional control of the competence factor βFTZ-F1 by juvenile hormone in the mosquito Aedes aegypti
Posttrapscriptional control of the competence factor βFTZ-F1 by juvenile hormone in the mosquito Aedes aegypti
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DOI：
10.1073/pnas.2234416100
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发表时间：
2003-11-11
影响因子：
11.1
通讯作者：
Raikhel, AS
中科院分区：
文献类型：
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作者：
Zhu, JS;Chen, L;Raikhel, AS
在自体蚊子中，卵黄发生，包括卵黄蛋白前体的产生，需要吸血。因此，蚊子传播许多疾病。了解卵黄发生调控的分子机制将有助于媒介控制策略的制定。新出现的埃及伊蚊雌性需要3天才能对血粉引发的20-羟基蜕皮激素（20 E）滴度升高做出反应，从而激活卵黄发生。孤儿核受体基因betaFTZ-F1在新出现的雌性蚊子的脂肪体中转录;然而，betaFTZ-F1蛋白仅在3天后被发现。保幼激素III（JH III）滴度的显著增加对于获得20 E能力至关重要。体外脂肪体培养实验表明，暴露于JH III后出现betaFTZ-F1蛋白。将与betaFTZ-F1互补的双链RNA注射到新出现的雌性中，响应于血餐，减弱了早期基因EcR-B、E74 B和E75 A以及靶YPP基因Vg的表达。因此，β FTZ-F1确实是定义蚊子脂肪体中获得对20 E的感受态的因子。此外，这是通过JH III介导的β FTZ-F1的转录后控制实现的。
In anautogenous mosquitoes, vitellogenesis, which includes production of yolk protein precursors, requires blood feeding. Consequently, mosquitoes transmit many diseases. Understanding the molecular mechanisms of vitellogenesis regulation will contribute significantly to vector control strategies. Newly emerged Aedes aegypti females require 3 days before becoming competent to activate vitellogenesis in response to a blood-meal-initiated, elevated titer of 20-hydroxyecdysone (20E). An orphan nuclear receptor gene betaFTZ-F1 is transcribed in the fat body of newly emerged mosquito females; however, the betaFTZ-F1 protein is only found 3 days later. Dramatically increased titer of the juvenile hormone III (JH III) is essential for the acquisition of 20E competence. In vitro fat body culture experiments have shown that betaFTZ-F1 protein appears after exposure to JH III. Injection of double-stranded RNA complementary to betaFTZ-F1 into newly emerged females attenuated expression of the early genes EcR-B, E74B, and E75A and the target YPP gene Vg, in response to a blood meal. Thus, betaFTZ-F1 is indeed the factor defining the acquisition of competence to 20E in the mosquito fat body. Moreover, this is achieved through JH Ill-mediated posttranscriptional control of betaFTZ-F1.