Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from glucose
Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from glucose
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DOI：
10.1007/s00253-015-6380-0
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发表时间：
2015-03-01
影响因子：
5
通讯作者：
Qi, Qingsheng
中科院分区：
文献类型：
--
作者：
Wang, Qian;Luan, Yaqi;Qi, Qingsheng
共聚物聚(3-羟基丁酸酯-3-羟基己酸酯)[P(HB-co-HHx)]具有作为可生物降解组织工程材料的潜力。然而，这种共聚物的生产仍然存在成本高和不确定性大的问题。我们在这里描述了利用重组大肠杆菌从葡萄糖直接合成P(HB-co-HHx)的代谢途径的设计。通过将BktB依赖的缩合途径和反向β氧化循环途径相结合，我们能够在重组大肠杆菌中合成HHx含量为10mol%的P(HB-co-HHx)共聚物。经过对宿主菌的优化和硫酯酶突变株的筛选，工程菌产生了12.9wt%的P(HB-co-HHx)，3HHx的摩尔分数为13.2mol%。
The copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] has the potential to serve as a biodegradable tissue engineering material. However, the production of this kind of copolymer still suffers from high cost and uncertainty. We describe here the design of metabolic pathways to synthesize P(HB-co-HHx) directly from glucose using recombinant Escherichia coli. By combining the BktB-dependent condensation pathway with the inverted beta-oxidation cycle pathway, we were able to synthesize a P(HB-co-HHx) copolymer with a 10 mol% HHx fraction in recombinant E. coli. After optimizing the host strain and employing thioesterase mutant strains, the engineered E. coli produced 12.9 wt% P(HB-co-HHx) with a 13.2 mol% 3HHx fraction.