Role of Surface Negative Charge in Platelet Function Related to the Hyperreactive State in Estrogen-Treated Prostatic Carcinoma
Role of Surface Negative Charge in Platelet Function Related to the Hyperreactive State in Estrogen-Treated Prostatic Carcinoma
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DOI：
10.1055/s-0038-1657168
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发表时间：
1982-06
影响因子：
6.7
通讯作者：
Stephanie M Jung;K. Kinoshita;K. Tanoue;I. Isohisa;H. Yamazaki
中科院分区：
文献类型：
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作者：
Stephanie M Jung;K. Kinoshita;K. Tanoue;I. Isohisa;H. Yamazaki
通过测定前列腺癌、前列腺癌合并雌激素、前列腺癌合并雌激素、前列腺癌合并阿司匹林、前列腺肥大和健康老年男性的血小板电泳率(EPM)、聚集性和唾液酸，探讨了血小板表面负电荷与功能亢进的关系。经雌激素治疗的前列腺癌患者的血小板EPM显著升高。唾液酸与红细胞压积呈良好的线性相关(r=0.97p<；0.001)。EPM与肾上腺素和ADP的一次聚集呈负相关，与二次或最大聚集无关，提示表面负电荷增加可能抑制一次聚集。雌激素和血小板数量的变化影响表面负电荷。神经氨酸酶去除血小板表面唾液酸导致EPM呈剂量依赖性下降，与唾液酸下降平行。阿司匹林治疗的患者和与阿司匹林体外孵育的血小板均显示血小板EPM增加。这些结果提示，血小板表面负电荷可能直接影响血小板功能。
Summary The relationship between platelet surface negative charge and hyperfunction was examined by determining electrophoretic mobility (EPM), aggregability, and sialic acid of platelets in prostatic cancer, prostatic cancer with estrogen, prostatic cancer with estrogen and aspirin, prostatic hypertrophy, and healthy aged males. Estrogen treated prostatic cancer patients had significantly higher platelet EPM. A good linear correlation was found between sialic acid and EPM (r = 0.97, p <0.001). EPM was negatively correlated with primary aggregations by adrenaline and ADP but not with secondary or maximum aggregations, suggesting increased surface negative charge may inhibit primary aggregation. Estrogen and platelet population changes influenced surface negative charge. Neuraminidase removal of platelet surface sialic acid resulted in dose-dependent decreases of EPM which paralleled decreases in sialic acid. Aspirin treated patients and platelets incubated with aspirin in vitro both showed increased platelet EPM. These results suggest that platelet surface negative charge may directly affect platelet function.