Conserved vacuolar H+-ATPase subunit B1 improves salt stress tolerance in apple calli and tomato plants
Conserved vacuolar H+-ATPase subunit B1 improves salt stress tolerance in apple calli and tomato plants
复制标题
保守的液泡H-ATP酶亚基B1提高苹果愈伤组织和番茄植株的盐胁迫耐受性
DOI：
10.1016/j.scienta.2015.09.019
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发表时间：
2015-12-14
影响因子：
4.3
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
--
作者：
Hu, Da-Gang;Sun, Mei-Hong;Hao, Yu-Jin
极性H ~+-ATPase（V-H ~+-ATPase）是一种高度保守的多亚基质子泵，在植物适应非生物胁迫过程中起着重要作用。苹果V-ATP酶亚基B1（MdVHA-B1）在植物耐旱性中的功能已被证实，但其是否参与植物的耐盐性尚不清楚。本研究对MdVHA-B1基因在盐胁迫应答中的功能进行了初步研究。GUS和Western blotting分析表明，MdVHA-B1在盐胁迫下受到了强烈的诱导。酵母双杂交（Y2 H）和双分子荧光互补（BiFC）分析表明，苹果中MdVHA-B1与拟南芥SOS 2样蛋白激酶MdSOS 2L 1相互作用。与野生型苹果愈伤组织相比，盐胁迫下转基因苹果愈伤组织的V-ATPase活性显著提高。此外，Na+和K+在转基因番茄植株中的积累比WT对照高得多。这些结果表明，MdVHA-B1可能通过盐过度敏感（SOS）信号通路调节离子平衡赋予苹果耐盐性。(C)2015爱思唯尔B. V.保留所有权利。
Vacuolar H+-ATPase (V-H+-ATPase) is highly conserved, multisubunit proton pumps that play key roles in adaptation to abiotic stress in plants. Function of apple V-ATPase subunit B1 (MdVHA-B1) has been identified in drought tolerance, however, it remains unclear whether MdVHA-B1 is involved in salt tolerance. In this work, we characterized the function of MdVHA-B1 gene in salt stress response. GUS and Western blotting assays showed that MdVHA-B1 was highly induced by salt stress. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays demonstrated that MdVHA-B1 interacts with MdSOS2L1, a Arabidopsis SOS2-like protein kinase, in apple. Compared with wild-type (WT) apple calli, V-ATPase activities in the transgenic calli were significantly enhanced under salt stress condition. Furthermore, Na+ and K+ were accumulated much higher in transgenic tomato plants than the WT control. These results suggest that MdVHA-B1 may confer salt tolerance through salt overly sensitive (SOS) signal pathway in apple to regulate the ion balance. (C) 2015 Elsevier B.V. All rights reserved.