Monoclonal hybridoma anti-acetylcholine receptor antibodies: antibody specificity and effect of passive transfer.
Monoclonal hybridoma anti-acetylcholine receptor antibodies: antibody specificity and effect of passive transfer.
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单克隆杂交瘤抗乙酰胆碱受体抗体：抗体特异性和被动转移效应。
DOI：
10.1111/j.1749-6632.1981.tb33726.x
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发表时间：
1981
影响因子：
5.2
通讯作者：
Fitch,FW
中科院分区：
文献类型：
--
作者：
Gomez,CM;Richman,DP;Burres,SA;Arnason,BG;Berman,PW;Fitch,FW
有令人信服的证据表明，乙酰胆碱受体（AChR）的抗体是负责重症肌无力（MG）的症状和神经肌肉病理。令人惊讶的是，血清抗体滴度与疾病的严重程度相关性很低。这种看似不一致的基础可能在于血清抗AChR抗体的几种特性，已知这些特性因患者而异。抗体结合的AChR抗原位点的差异，如针对α银环蛇毒素（aBT）结合位点2的抗体的百分比所示，以及它们与不同物种AChR交叉反应的程度9已被报道。抗AChR抗体的免疫球蛋白类别和亚类也已知是变化的。最后，它们在生物学性质上存在差异，例如它们对AChR功能的影响，诱导AChR调节的能力，以及将肌无力被动转移到小鼠的能力。已经提出，这些特性中的一个或多个的变化可能解释抗AChR抗体滴度与疾病严重程度之间的不良相关性，即某些抗AChR抗体滴度与疾病严重程度之间的相关性。
Compelling evidence exists that antibodies to acetylcholine receptor (AChR) are responsible for the symptoms and neuromuscular pathology in myasthenia gravis (MG). l Surprisingly, serum antibody titer correlates poorly with disease se~ erity.~-~ The basis for this seeming discrepancy may possibly lie in the several properties of serum anti-AChR antibodies which are known to vary from patient to patient. Differences in the AChR antigenic sites to which antibodies bind, as seen both by the percent of antibodies directed against the alpha bungarotoxin (aBT) binding site 2, and the degree to which they crossreact with AChR of different species 9 have been reported. The immunoglobulin classes and subclasses of the anti-AChR antibodies are also known to vary. lO Finally there are differences in biologic properties such as in their effect on AChR function, ll, l 2 ability to induce modulation of AChR, 13--’5 and ability to passively transfer myasthenia to mice. lC It has been proposed that variation in one or more of these properties might account for the poor correlations between anti-AChR antibody titer and disease severity, ie, certain anti-AChR