Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets
Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets
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DOI：
10.1371/journal.pone.0235052
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发表时间：
2020-06-25
期刊：
影响因子：
3.7
通讯作者：
Wszola, M.
中科院分区：
文献类型：
--
作者：
Klak, M.;Gomolka, M.;Wszola, M.
前言3D打印在现代生物医学中的应用越来越广泛。其中一个问题是选择合适的生物印花材料的交联方法。在目前使用的技术中，我们可以区分：物理交联法(例如，Ca(2+)和Sr2+)和化学交联法--引起最大讨论的紫外光交联法。UV辐射被DNA选择性地吸收，主要在UV-B区，但也(在一定程度上)在UV-A和UV-C区。DNA的兴奋导致了典型的照片产品。链断裂的数量可能会因暴露时间的不同而不同，当细胞在辐射后孵化时，也可能不同。目的研究405 nm和365 nm两种波长的照射时间是否以及如何影响细胞和微器官(胰岛)的DNA损伤。材料与方法用彗星试验评价不同波长(405 nm和365 nm)辐射对DNA的损伤程度。在孵育24小时后，对成纤维细胞、α细胞、β细胞和猪胰岛进行检测。选择未经放射治疗的标本作为对照组。结果分析包括DNA损伤细胞百分率的测定和尾部强度评价。结果405 nm波长照射后，胰岛细胞DNA损伤程度随实验时间(10~300s)变化，在2%~6%之间波动。然而，用365 nm波长处理胰岛会导致高达50%的损伤。这清楚地表明，当使用405 nm波长时，损害明显较小。被测试的细胞系也得到了类似的结果。结论405 nm紫外光对胰岛细胞的作用优于365 nm紫外光的交联剂。
Introduction 3D printing is being used more extensively in modern biomedicine. One of the problems is selecting a proper crosslinking method of bioprinted material. Amongst currently used techniques we can distinguish: physical crosslinking (e.g. Ca(2+)and Sr2+) and chemical crosslinking-the UV light crosslinking causing the biggest discussion. UV radiation is selectively absorbed by DNA, mainly in the UV-B region but also (to some extent) in UV-A and UV-C regions. DNA excitement results in typical photoproducts. The amount of strand breaks may vary depending on the period of exposition, it can also differ when cells undergo incubation after radiation. Aim The aim of this study was to show whether and how the time of irradiation with 405 nm and 365 nm wavelengths affect DNA damage in cell lines and micro-organs (pancreatic islets). Materials and methods The degree of DNA damage caused by different wavelengths of radiation (405 nm and 365 nm) was evaluated by a comet assay. The test was performed on fibroblasts, alpha cells, beta cells and porcine pancreatic islets after 24 hours incubation period. Samples without radiation treatment were selected as a control group. Results analysis consisted of determining the percent of cells with damaged DNA and the tail intensity evaluation. Results The degree of DNA damage in pancreatic islets after exposure to 405 nm wavelength oscillated between 2% and 6% depending on the tested time period (10 - 300 seconds). However, treating islets using 365 nm wavelength resulted in damage up to 50%. This clearly shows significantly less damage when using 405 nm wavelength. Similar results were obtained for the tested cell lines. Conclusions Crosslinking with 405 nm is better for pancreatic islets than crosslinking with 365 nm UV light.